摘要
Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.
Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第5期475-477,共3页
Acta Microbiologica Sinica
基金
国家自然科学基金
关键词
类产碱假单胞菌
谷氨酸脱氢酶
纯化和性质
Pseudomonas pseudoalcaligenes,Glutamate dehydrogenase,Purification and Property
