胶质瘤细胞增殖与凋亡的研究

A STUDY OF THE CELL PROLIFERATION AND APOPTOSIS IN GLIOMAS

对未经其他治疗的手术摘除的胶质瘤标本，采用免疫组化和原位标记方法研究了胶质瘤及其瘤旁组织中细胞增殖以及凋亡的状况。结果表明，增殖细胞核抗原在世界卫生组织（ＷＨＯ）制定的不同级别的胶质瘤中其阳性率具有显著性差异（Ｐ＝ ０．０００５），而在胶质瘤与其瘤旁组织之间则无显著性差异。恶性程度不同的胶质瘤中细胞发生凋亡的比率具有显著性的差异（Ｐ＝ ０．０１７０），而在胶质瘤与其瘤旁组织间无显著性差异。增殖细胞核抗原是划分胶质瘤恶性程度的一个较好的指标，与Ｗ ＨＯ 胶质瘤恶性程度分级具有较好的相关性（ｒ＝ ０．４０８９）。Ｗ ＨＯ Ⅱ级星形胶质瘤细胞凋亡明显增多，可能是保留了恶性程度较高的胶质瘤细胞而清除了恶性程度相对较低的胶质瘤细胞，从而可能促进了胶质瘤的恶性进展。胶质瘤邻近的瘤旁组织可能已具有了胶质瘤的某些特性，胶质瘤呈浸润性生长可能与此有密切的关系。

The purpose of this report is to analyse the relationship of cell proliferation and apoptosis within gliomas. 75 cases of Gliomas from surgery were studied by using immunohistochemistry and in situ hybridization. The results showed that the ratio of PCNA immunoreactive positive cells was statistically different among gliomas of the four WHO grades(P=0.0005). There was no statistical difference between gliomas and their surrounding tissue. For TUNEL positive cells, the ratio was statistically different between every two WHO grade gliomas(P=0.0170), and there was no statistical difference between gliomas and their surrounding tissue also. These results suggest that the cell proliferation marker PCNA is a helpful indicator to grade the malignancy of gliomas. The malignant transformation of gliomas might be accelerated by apoptosis of the comparatively normal phenotype tumor cells. The surrounding tissue of gliomas may have been of some character of gliomas and may be have close relationship with their aggression. (Figures 1～6 on plate 44)