肿瘤细胞对腺病毒载体转染的敏感性研究

SENSITIVITY OF TUMOR CELLS TO TRANSFECTION OF ADENOVIRUS VECTOR

带有基因LacZ的5型缺陷性腺病毒感染肿瘤细胞，X－gal染色，检测腺病毒对肿瘤细胞的转染率。结果显示，随着病毒感染复数量（multiplicityofinfection，m.o．i）的增加，转染率也增高，且无细胞毒作用。各种肿瘤达到100％转染所需病毒量是不同的，人类肿瘤细胞系100％转染所需的m.o．i分别是：Hela细胞为100，GRC肾癌细胞为200，Hep-2喉癌细胞为200，鼠肿瘤所需病毒量较大，Lewis肺癌细胞为500，BST膀胱癌细胞为600。说明不同肿瘤细胞对腺病毒载体转染的敏感性是不同的。

Tumor cells were transfected by AdCMLacZ. The number of transfected cells was assessed by X-gal staining. The results showed that as the MOI (multiplicity of infection) increases the transfection efficiency also incr eases, and no CPE exists. Different tumor cells need different MOI of AdCMLacZ to get 100%transfection. Hea cells need 100, GRC kidney cancer cells need 200, Hep-s larynx cancer cells need 200.While mice tumor cells need even more, Lewis lung cancer cells need 500; BST bladder cancer cells need 600. These indicate that the senstivity of different tumor cells to transfection of adenovirus vector was different.