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原因不明自然流产发病机制的研究 被引量:6

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摘要 目的通过对正常妊娠模型小鼠CBA×BALB/c与自然流产模型小鼠CBA×DBA/2某些生物学特性对比研究,探讨胎盘单位免疫异常、细胞增生和凋亡失衡以及细胞的识别和黏附能力下降或缺陷等与原因不明自然流产之间的关系。方法建立正常妊娠小鼠模型CBA×BALB/c和自然流产小鼠模型CBA×DBA/2;采用免疫组化法检测两组模型绒毛滋养层和蜕膜组织中细胞因子与受体([肿瘤坏死因子-α(TNF-α)及其受体(TNFR1)、单核细胞趋化蛋白-1(MCP-1)、凋亡调控蛋白Bcl-2、Bax、Fas、FasL和细胞增殖与黏附相关蛋白PCNA、OPN、Survivin)的表达;采用酶联免疫吸附试验(ABC-ELISA)测定两组模型血清sTNFR的表达,采用DNA缺口原位末端标记技术(TUNEL)测定两组模型绒毛滋养层和蜕膜组织细胞凋亡。结果自然流产模型蜕膜组织中TNF-α、MCP-1和TNFR1均显著高于正常妊娠模型,血清sTNFR亦显著高于正常妊娠模型;绒毛滋养层和蜕膜组织中细胞凋亡指数自然流产模型明显高于正常妊娠模型;自然流产模型绒毛滋养层和蜕膜组织中PCNA、OPN和Survivin表达均低于正常妊娠模型。结论原因不明自然流产的发生与胎盘单位免疫异常、细胞的增生和凋亡失衡以及细胞的识别和黏附能力下降缺陷有一定关系。
作者 米亚英
出处 《中国医药科学》 2011年第1期24-25,54,共3页 China Medicine And Pharmacy
基金 国家人口计生委科技司立项(课题编号:C1-41)
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参考文献8

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