通腑泻肺法对ALI/ARDS大鼠血清炎症因子表达的影响

The Effect of Tongfu Xiefei-therapy on the Expression of Serum Inflammatory Factors in ALI/ARDS Rats

目的观察通腑泻肺法对ALI/ARDS大鼠血清炎症因子的影响,探讨ALI/ARDS肺肠同治的生物学基础。方法将100只健康Wistar大鼠随机分为5组:空白对照组、模型组、治肺组、治肠组和肺肠同治组,采用尾静脉注射油酸加内毒素方法造成大鼠ALI/ARDS模型,观察大鼠血清TNF-α、IL-1β、IL-6、IL-10含量。结果通腑泻肺法可以有效降低大鼠全身炎性因子TNF-α、IL-1β、IL-6、IL-10表达水平。结论 (1)通腑泻肺法能够明显减轻ALI/ARDS大鼠免疫炎症损伤,其保护作用较单纯用泻肺法或通腑法效果更有优势;(2)泻肺法、通腑法和通腑泻肺法对ALI/ARDS大鼠血清炎性因子作用靶点不同。

Objective To observe the effect of Tongfu Xiefei-therapy on ALI/ARDS rats＇ autoimmune inflammatory damage and in- vestigate the biological basis of treating the lung and the large intestine at the same time. Methods We divided 100 healthy wistar rats into 5 groups：control group,model group,lung treatment group,large intestine treatment group and lung-large intestine treatment group. We made the ALI/ARDS-rat model by intravenous injection of oleic acid plus endotoxin in caudal vein,and observed TNF-a, IL-1β ,IL-6,IL-10 in serum. Results Tongfu Xiefei-therapy could effectively reduce the expression of TNF-a, IL-1 β ,IL-6,IL-10 in serum of ALI/ARDS rats. Conclusion （1）Tongfu Xiefei-therapy could effectively reduce autoimmune inflammatory in ALI/ARDS rats,and the combination method was superior than either single method of Xiefei therapy or Tongfu therapy; （2）The action targets of Xiefei therapy,Tongfu therapy and Tongfu Xiefei-therapy on inflammatory cvtokines in ALI/ARDS rats serum were different.