摘要
目的 探讨鼻腔吸入γ干扰素（interferon gamma,IFN-y）对大鼠变应性鼻炎（allergicrhinitis,AR）转化生长因子β1（transforming growth factor-β1,TGF-β1）/Smad信号通路的影响.方法 采用卵白蛋白、氢氧化铝建立大鼠AR鼻黏膜重塑模型.分为AR模型组（B组）、IFN-γ组（C组）,每组10只大鼠,分别于第4～10周,每周2次每只每侧鼻腔滴入磷酸盐缓冲液50μl、IFN-γ 1μg,另设阴性对照组（A组）大鼠10只,于最后一次吸入结束后24 h取鼻黏膜组织检测TGF-β1蛋白及TGF-β1、Smad2、Smad3、Smad7 mRNA的表达.结果 C组鼻腔黏膜组织中TGF-β1、Smad2、Smad3mRNA相对表达强度分别为0.59±0.04、0.39±0.08、0.46±0.15,明显低于B组的0.82±0.12、0.70±0.18、0.95±0.26,差异有统计学意义（q值分别为3.15、4.47、3.03,P值均〈0.05）;C组鼻腔黏膜组织中Smad7 mRNA相对表达强度为0.31±0.05,明显高于B组的0.25±0.06,差异有统计学意义（q=2.98,P〈0.05）.免疫组化显示B组鼻腔黏膜组织中TGF-β1蛋白表达增加,而C组的表达减少.结论 鼻腔吸入IFN-γ通过抑制AR大鼠鼻黏膜组织TGF-β1、Smad2、Smad3的过度表达,上调Smad7的表达,从而阻断TGF-β1/Smad信号通路,明显减轻了大鼠AR鼻黏膜的重塑.
Objective To investigate the effects of intranasal interferon gamma （IFN-γ） on nasal mucosa remodeling and expression of transforming growth factor-β1 （ TGF-β1 ）, Smad2, Smad3, Smad7 in allergic rhinitis （AR） rat model. Methods Ovalbumin （OVA） and aluminum hydroxide were used to construct the AR model. Thirty AR rats were randomly divided into positive control group （group B, n =10）, IFN-γ treatment group（ group C, n = 10） and negative control group（ normal rats, n = 10）. After the AR models were built, 50μl PBS, 1 μg IFN-γ was dropped into the nasal cavity of each rat in group B and group C, from the fouth week to tenth week, twice a week. The nasal mucosa was collected on day 71 in order to observe the pathologic changes, and the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA,Smad3 mRNA and Smad7 mRNA by immunohistochemistry and reverse transcriptase-polymerase chain reaction. Results Decreases of TGF-β1, Smad2 and Smad3 mRNA were seen in nasal tissue of group C （0. 59 ±0. 04, 0. 39 ±0. 08, 0. 46 ±0. 15） as compared with group B （0. 82 ±0. 12, 0. 70 ±0. 18, 0. 95 ±0. 26） , the differences were significant （ q value were 3.15, 4. 47, 3.03, all P 〈 0. 05 ）. The levels of Smad7 mRNA expression increased significantly （ q = 2.98, P 〈 0. 05 ） in group C （ 0. 31 ± 0. 05 ） as compared with group B （ 0.25±0.06）. Immunohistochemistry showed significant decrease of TGF-β1expression in the nasal tissue of group C much lesser than that in group B. Conclusions Intranasal IFN-γcould decrease the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA, Smad3 mRNA, increase the expression of Smad7 mRNA in AR rats model and inhibit the nasal mucosa remodeling.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2011年第1期59-62,共4页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
