独角莲超临界萃取物的GC-MS分析及体外抑瘤活性

GC-MS Analysis and in Vitro Antitumor Activity of the Extract from Typhonium giganteum Engl using Supercritical Fluid CO_2

为了寻找独角莲抗肿瘤的活性物质基础和合理开发利用独角莲,本研究采用超临界CO2萃取方法提取独角莲块茎粉,通过气相色谱—质谱技术对提取物进行成分鉴定。并采用噻氮唑蓝比色法(MTT)测试萃取物的体外抑瘤活性。结果:从独角莲的超临界CO2萃取物中检出37种物质,含量最丰富的4种化合物为:β-谷甾醇(-βS itos-terol),占40.22%;菜油甾醇(Campesterol),占18.45%;棕榈酸(n-Hexadecanoic acid),占9.52%;亚油酸(9,12-Oc-tadecadienoic acid(Z,Z)-),占8.15%。MTT实验证明萃取物对结肠癌HCT-8、卵巢癌HO-8910、胃癌SGC-7901、肝癌SMMC-7721均有显著的抑制作用,其中肝癌SMMC-7721细胞对萃取物最为敏感。独角莲块茎超临界CO2萃取物有显著抑瘤活性,针对该萃取物的进一步分离分析对其活性物质基础的寻找具有重要意义。

In order to explore the antitumor active components of Typhonium giganteum Engl,the powder of T.giganteum Engl tubers was extracted using supercritical fluid CO2-80% ethanol,and the extract was identified by gas chromatography-mass spectrometry（GC-MS） technology.The in vitro antitumor effects of the extract was evaluated by MTT colorimetric method.Thirty-seven compounds were identified from the extract by GC-MS analysis.Four major components are as follows： β-Sitosterol,accounting for 40.22%;Campesterol,accounting for 18.45%;n-Hexadecanoic acid,accounting for 9.52%;9,12-Octadecadienoic acid（Z,Z）-,accounting for 8.15%.MTT experiments indicated that the extract showed significant inhibition on the colon cancer HCT-8,ovarian cancer HO-8910,gastric cancer SGC-7901,and liver cancer SMMC-7721 cells.Liver cancer SMMC-7721 cells were the most sensitive to the extract.The extract of T.giganteum Engl tubers by supercritical fluid CO2 showed remarkable anti-tumor effect.Further research on this extract is necessary to find its active substances for reasonable development and utilization of T.giganteum Engl.