人参皂苷CK对胃癌细胞株SGC-7901及其内源性VEGF的影响

Effects of Ginsenoside CK on Gastric Cancer SGC-7901 Cell Line and Endogenous VEGF Secreted by Tumor Cells

目的研究人参皂苷CK对胃癌SGC-7901细胞增殖、细胞周期的影响及其对内源性分泌的血管内皮细胞生长因子(VEGF)的作用。方法以50、25、12.5、6.25、3.125、1.5625μg/ml的人参皂苷CK作用于胃癌细胞株SGC-7901,通过MTT法检测人参皂苷CK对细胞的抑制作用;采用流式细胞术检测细胞周期和细胞凋亡;ELISA定量检测细胞培养液中内源性VEGF的含量变化。结果 MTT法显示人参皂苷CK对胃癌细胞株SGC-7901有抑制作用,并且呈浓度、时间依赖关系;SGC-7901细胞经人参皂苷作用后出现明显凋亡峰,且细胞周期被阻滞在G0/G1期;人参皂苷CK处理组VEGF含量低于对照组(P<0.05),高浓度组低于低浓度组(P<0.05),且随着作用时间延长,VEGF含量降低。结论人参皂苷CK可通过诱导凋亡抑制胃癌细胞株SGC-7901生长,并可抑制SGC-7901细胞内源性分泌VEGF,人参皂苷CK可能成为一种潜在的抗胃癌药物。

Objective To investigate the effects of Ginsenoside CK on the cell proliferation、cell cycle and endogenous VEGF of gastric cancer SGC-7901 cell line.Methods SGC-7901 cell line in cultural medium in vitro were given 50,25,12.5,6.25,3.125,1.5625 μg/ml Ginsenosde CK.The inhibitory rate of cell line was measured by MTT assay;cell apoptotic rate and cell phase were detected by flow Cytometry（FCM）;the VEGF contents were detected by ELISA assay.Results It was found that Ginsenoside CK had significant inhibitory effect on the proliferation of SGC-7901 cell line,and this effect was dose-and-time-dependent;FCM assay indicated that apoptosis peak appeared evidently after the cells being interfered with Ginsenoside CK and most of cells were arrested in G0/G1 phase;The levels of VEGF in Ginsenoside CK groups were lower than that in the control group（P0.05）.The level of VEGF in the groups of high concentration Ginsenoside CK were lower than that in the group of low concentration（P0.05）,and the VEGF contents decrease with action time prolonging.Conclusion Ginsenoside CK dramatically inhibits proliferation of SGC-7901 cell line by inducing cell apoptosis and secretion of endogenous VEGF.Ginsenoside CK may become a potential drug in the prevention and treatment of gastric cancer.