子宫内膜样腺癌组织中p27、MCM7蛋白的表达及意义

Expression and Significance of p27 and MCM7 Proteins in Endometrioid Adenocarcinoma

目的探讨p27、MCM7蛋白在子宫内膜样腺癌及其癌前病变组织中的表达及意义。方法采用免疫组织化学SP法检测30例正常子宫内膜、30例内膜增生、30例非典型增生和50例子宫内膜样腺癌组织中p27和MCM7蛋白的表达情况。结果 p27蛋白的阳性表达水平在正常子宫内膜、内膜增生、非典型性增生到子宫内膜样腺癌组织中呈逐渐下降趋势,组间两两比较差异均有统计学意义(P<0.05);而MCM7阳性表达水平呈逐渐上升趋势,子宫内膜样腺癌组和非典型增生组均显著高于正常组和内膜增生组(P<0.05),子宫内膜样腺癌组中p27与MCM7的表达呈负相关(r=-0.3306,P<0.02),p27蛋白低表达与组织学分级及手术病理分期有关(P<0.05),MCM7蛋白的高表达与组织学分级、肌层浸润、淋巴结转移和手术病理分期有关(P<0.05);p27低表达和MCM7的高表达与肿瘤的复发有关(P<0.05)。结论 p27蛋白的表达缺失和MCM7的高表达可能涉及了子宫内膜样腺癌的发生、发展过程,两者的联合检测可望作为临床早期诊断、评价预后的生物学指标。

Objective To study the expression of p27 and MCM7 protein in endometrioid carcinoma and its clinical significance.Methods The expression of p27 and MCM7 proteins were tested by SP immunohistochemistry from normal endometrium（30 cases）,endometrial hyperplasia（30 cases）,atypical hyperplasia（30 cases） and endometrioid carcinoma（50 cases）.Results The positive expression of p27 decreased gradually in different groups：normal endometrium endometrial hyperplasia endometrial dysplasia endometrioid carcinoma.There was a significant difference amory these groups（P0.05）.But the positive expression of MCM7 in four groups showed an increased gradually.The expression of MCM7 in endometrioid carcinoma and endometrial dysplasia groups were significently higher than those in normal endometrium and endometrial hyperplasia groups（P0.05）.Negative correlation was found between the expression of p27 and MCM7（r=-0.3306,P0.02）.In endometrioid carcinoma group,the lower expression of p27 protein was significantly associated with histological grading（P0.05）and pathologic stage（P0.05）.The overexpression of MCM7 protein was significantly correlated with histological grading,myometrial invasion,lymph node metastasis and pathologic stage（P0.05）,respectively.The lower expression of p27 and higher expression of MCM7 were related with recurrence in endometrioid carcinoma（P0.05）.Conclusion The results suggest that the low expression of p27 and the overexpression of MCM7 protein may be involved in carcinogenesis and development of medometrioid carcinoma.Combined examining of p27 and MCM7 protein may be used as a biomarker in the early diagnosis and for the prognosis judgement of endometrioid carcinoma.