糖尿病视网膜病变患者房水VEGF和IL-6水平的测定及其临床意义

Detection of levels of VEGF and IL-6 in the aqueous humor of patients with diabetic rctinopathy and its clinical significance

目的 探讨糖尿病视网膜病变（DR）程度与房水中血管内皮生长因子（VEGF）和白细胞介素-6（IL-6）含量之间的关系.方法 采用双抗体夹心酶联免疫吸附（EIISA）法,测定88例房水中VEGF和IL-6的含量,根据散瞳眼底检查和眼底荧光素血管造影检查后,实验组分为：无糖尿病视网膜病变组（NDR）21例、单纯型糖尿病性视网膜病变组（BDR）27例、增生型糖尿病性视网膜病变组（PDR）20例;对照组为健康的老年性白内障患者20例.结果 NDR组、BDR组、PDR组的房水VEGF含量分别为（240.29±26.14）pg/ml、（292.32±58.43）pg/ml、（477.65±90.08）pg/ml,IL-6含量分别为（160.81±33.40）pg/ml、（238.82±62.01）pg/ml、（389.42±90.08）pg/ml;对照组房水VEGF含量为（140.56±26.24）pg/ml、IL-6含量为（82.74±21.51）pg/ml,对照组与实验组比较差异均有统计学意义（F=113.67,P〈0.01;F=106.53,P〈0.01）.各组房水VEGF和IL-6含量有明显增加的趋势.房水中的VEGF含量与相应的IL-6含量有统计学意义（r=0.995,P〈0.01）;房水中VEGF、IL-6含量与糖尿病患者的病程及糖尿病视网膜病变的严重程度差异有统计学意义（r=0.722,P=0.01;r=792,P〈0.01）、（r 0.869,P〈0.01;r=0.865,P〈0.01）.结论 VEGF、IL-6在糖尿病视网膜病变的形成过程中有重要作用,且两者之间有相关性.

Objective To explore the relationship between diabetic retinopathy （DR） and vascular endothelial growth factor （VEGF）, interleukin-6 （IL-6） levels in the aqueous humor. Methods Aqueous humor samples were collected from diabetic patients with cataracts or without retinopathy, and aqueous humor samples from non-diabetic patients with cataracts were chosen as controls. The content of VEGF in the aqueous humor was determined by using the ELISA technique. Results The level of VEGF in the aqueous humor samples of diabetic patients without retinopathy （NDR）, background diabetic retinopathy （BDR） and proliferative diabetic retinopathy （PDR） were （240.29± 26.14） pg/ml, （292.32± 58.43） pg/ml and （477.65± 90.08） pg/ml respectively. The level of IL-6 in the aqueous humor from NDR, BDR and PDR were （160.81± 33.40） pg/ml, （238.82±62.01） pg/ml and （389.42± 90.08） pg/ml respectively. The levels of VEGF and IL-6 in the aqueous humor from the control group were （140.56± 26.24） pg/ml, （82.74± 21.51） pg/ml respectively. Compared with the control,the level of VEGF and IL-6 in the aqueous humor of diabetic patients increased significantly （P〈0.01）. The level of VEGF and IL-6 in the aqueous of the NDR, BDR and PDR also increased significantly （P 〈0.01）. The level of VEGF significantly correlated with the level of IL-6 in the aqueous humor （r=0.995, P〈0.01）. The level of VEGF and IL-6 in the aqueous humor also significantly correlated with the severity and prolongation of DR （r=0.869, P 〈0.01 ;r=0.865, P 〈0.01）. Conclusions VEGF and IL-6 play active roles in the generation and development of DR, and the level of VEGF in the aqueous humor closely correlates with the level of IL-6 in the aqueous humor of diabetic patients.