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H3N2亚型犬流感病毒的分离鉴定 被引量:8

Isolation and identification of H3N2 subtype canine influenza virus
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摘要 为了解广州地区犬流感的流行情况,本研究采集107份犬鼻咽拭子样品和58份血清样品,SPF鸡胚分离病毒并进行抗体检测。结果显示:分离获得3株H3N2亚型犬流感病毒。分离株的EID50为10-2.42/0.1 mL~10-3.5/0.1 mL;MDT为177.6 h~192 h;对乙醚、氯仿、酸和温度均敏感;对血凝素为热不稳定型;能够凝集公鸡、豚鼠、猪和牛的红细胞。 To evaluate the prevalence of canine influenza virus (C1V) in Guangzhou area, samples of 107 nasopharynx swabs and 58 serum were collected from dogs for virus isolation and antibody detection. Three H3N2 subtype CIV were isolated. The 50% embryo infectious dose (EIDs0) of the three isolates were 10-242/0.1 mL to 10-35/0.1 mL respectively. The mean death time (MDT) ranged from 177.6 h to 192 h. All three isolates were sensitive to ether, chloroform acidum and heat, and the isolates possessed HA activity to erythrocytes of rooster, guineapigs, pig and cattle.
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2011年第1期15-18,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金(30972233) 广东省科技计划项目(2008A060202017、2009B060300024) 广东省自然科学基金重点项目(8251064201000008) 教育部博士点基金(20094404110017) 重要病毒跨种间感染与传播致病的分子机制研究(2011CB504700-1)
关键词 犬流感病毒 H3N2亚型 分离鉴定 生物学特性 CIV H3N2 subtype isolation and identification biological characterization
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