应用DNA芯片技术筛选禽致病性大肠杆菌和尿道致病性大肠杆菌的差异表达基因

DNA microarray analysis of differential gene expressions of avian pathogenic Escherichia coli and uropathogenic Escherichia coli in different growth conditions

为研究禽致病性大肠杆菌强毒株E058的毒力相关基因在鸡体内、体外表达情况以及E058和尿道致病性大肠杆菌HEC4在LB和尿液中培养的表达情况,本研究分别提取E058株在SPF鸡体内及E058株和HEC4株在LB和尿液中静置培养的总RNA,与构建的DNA芯片杂交,检测和分析RNA的差异表达情况。芯片的检测结果表明:E058株在鸡体内和LB中培养差异表达基因共有9个,上调基因为5个,分别为neuC、iutA、cvaC、aes-15和iucCD;下调基因为4个,分别为aes-8、gyrB、aec-30和mdh。另外,芯片检测结果也显示E058株和HEC4株在LB和尿液中静置培养,具有相似的基因表达情况。

To screen distinctively expressed genes of avian pathogenic Escherichia coli （APEC） 02 serotype E058 strain and uropathogenic Escherichia coli （UPEC） HEC4 strain, the mRNA from the E058 and HEC4 strains under different growth conditions were extracted and hybridized in DNA microarray chips, respectively. A total of 9 differential expressed genes of APEC E058 strain from inoculated chicken were identified when compared to those growth in LB broth, and five of them were up-regulated including icuCD, iutA, cvaC, neuC and aes-15 and 4 genes were down-regulated including aes-8, gyrB, aec-30 and mdh. The similar results of microarray were also found in UPEC HEC4 strain growth under urine and LB broth, respectively. The most of differential expressed genes were related to the virulence genes of APEC E058 and UPEC HEC4 strain.