副猪嗜血杆菌血清5型岳阳株对昆明小鼠淋巴器官损伤的研究

Lesion in lymphoid organs of Kunming mice experimentally inoculated with a Yueyang strain of Haemophilus parasuis serotype 5

为探讨副猪嗜血杆菌(HPS)对免疫器官的损伤,本实验将HPS血清5型岳阳株及其菌体裂解物分别接种昆明小鼠,结果显示:HPS血清5型岳阳株及其菌体裂解物,均能够引起昆明小鼠颈淋巴结细胞凋亡和坏死。组织病理学检测可见脾脏红髓扩张、白髓萎缩、淋巴细胞衰减;胸腺病变较轻,皮质部局灶性淋巴细胞坏死和散在性细胞凋亡。此外,免疫器官损伤与剂量相关,随菌量的增大病变更为明显。HPS血清5型岳阳株及其菌体裂解物对昆明小鼠淋巴器官有明显的损伤作用。

In order to explore the lesions of immune organs caused by Haemophilus parasuis infection, mice were experimentally inoculated with a Yueyang strain of H. parasuis serotype 5 and tissue lesions in the immune organs were examined. Cytonecrosis and apoptosis of lymphocytes in germinal center of cervical lymph node, expanding red pulp with white pulp atrophy, lymphocyte depletion of spleen, cytonecrosis of lymphocyte with scattered cells apoptosis in cortex of thymus could be observed following infection by H. parasuis serotype 5 and its lysate. The higher the dosage the bacterial culture or its lysate were inoculated, the more serious the lesions were developed. In conclusion, the Yueyang strain of H. parasuis serotye 5 and its lysate could cause severe pathological damage to the lymphatic organ of mice.