检测猪捷申病毒抗体的间接免疫荧光方法的建立与初步应用

Establishment of the indirect immunofluorescence assay for detecting the antibody of porcine teschovirus

为建立检测猪捷申病毒(PTV)抗体的方法,本研究利用PTV-8 Jilin/2003株为抗原,建立了检测PTV抗体的的间接免疫荧光技术(IFA)方法。该方法工作浓度分别是:一抗最适稀释度为1∶10;FITC标记二抗最适稀释度为1∶100;二抗中伊文氏兰最适稀释度为1∶30 000。敏感性试验、特异性试验表明该抗原板可以检出包含PTV-8型和1型在内的的所有PTV阳性血清,方法敏感、特异。与SN符合率94.0%。用该方法对黑龙江、山东、天津和河南等地的1 500份临床血清样品进行检测,阳性检出率为55.8%,表明我国猪群中PTV的感染已经非常普遍。该方法做为PTV的血清学诊断新技术,为开展PTV分子流行病学调查奠定了基础。

An indirect immunofluorescence assay （IFA） was established to detect porcine teschovirus （PTV） using PTV-8 Jilin/2003 as antigen. The assay could detect the serum at the 1：1,000 dilutions under optimized conditions, and had no cross reactions with antibodies against PPV, PRV, PRRSV, PCV2, CSFV and PEV. The IFA had 94.0% correlation with SN. A total of 1,500 serum samples from different farms were tested, and 55.8% detected positive, indicating that PTV infection was serious in China. The IFA was a convenient tool for the epidemiological investigation of PTV infections and evaluation of antibodies against the virus.