成都地区肥胖患者脂肪酸结合蛋白2基因Ala54Thr多态性研究

Analysis of the FABP2 Gene Ala54Thr Polymorphism in Non-obese and Obese Chinese

目的研究脂肪酸结合蛋白2基因(fattyacid-bindingprotein2,FABP2)Ala54Thr多态性是否与中国人肥胖有关联,为探讨肥胖的分子遗传基础提供依据。方法应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)对成都地区272例非肥胖者及121例肥胖患者FABP2基因Ala54Thr多态性位点进行分析,采用酶法和单向免疫扩散法对血脂和载脂蛋白水平进行测定。结果 FABP2基因Ala54Thr位点Ala、Thr等位基因的频率在肥胖组为71.5%、28.5%,在非肥胖组为71.1%、28.9%,两组间等位基因的频率差异无统计学意义(P>0.05)。Ala54Thr位点在肥胖组AlaThr基因型携带者血清甘油三酯(TG)水平高于AlaAla基因型者(P<0.05),进一步按性别分层后,肥胖组男性亚组存在此差异,且其AlaThr基因型者血清高密度脂蛋白胆固醇(HDL-C)水平低于AlaAla基因型者(P<0.05);在肥胖组女性及非肥胖组未见血脂与载脂蛋白水平差异存在统计学意义(P>0.05)。结论 FABP2基因Ala54Thr多态性与成都地区中国汉族人肥胖无关联,但与血清TG和HDL-C水平有关,且具有性别差异。

Objective To investigate the fatty acid-binding protein 2 gene（FABP2） Ala54Thr polymorphism and its relationship to obesity in Chinese population.Methods Three hundred and ninety three subjects（272 non-obese and 121 obese individuals） from a population of Chinese Han nationality in Chengdu area were studied using PCR-restriction fragment length polymorphisms（PCR-RFLPs）.Serum lipids were measured by enzymatic kits and apolipoproteins AⅠ,AⅡ,B100,CⅡ,CⅢ and E were measured by the RID kits.Results The frequencies of Ala and Thr allele at Ala54Thr site in obese and non-obese groups were 71.5%、28.5%,and 71.1%、28.9%,respectively.No significant difference in the allele frequencies between the two groups was observed（P〉0.05）.In the obese group,subjects with Thr allele（AlaThr ＋ThrThr genotype carriers）had higher serum triglyceride（TG） concentrations than those with genotype AlaAla（P〈0.05）.Similar results were observed in obese male subgroup,when male and female subgroups were further separated.In addition,obese males with AlaThr had lower HDL-C levels than those with genotype AlaAla.No significant difference of lipid and apolipoprotein levels was observed in obese females or non-obese group.Conclusion The Ala54Thr polymorphism in the FABP2 gene was not associated with obesity in Chinese Han population of Chengdu area.However,it may be associated with serum TG,HDL-C levels,with some gender-specific effect,in this population.