载脂蛋白AI基因+83C/T多态性对高糖膳食诱导的血清糖脂代谢指标变化的影响

Effects of the +83C/T Polymorphism in Apolipoprotein AI Gene on the Changes of Serum Biochemical Traits of Gluocse and Lipid Metabolism Induced by High-carbohydrate Diets

目的探讨载脂蛋白AI基因(apoA1)+83C/T多态性对高糖膳食诱导的血脂、血糖、胰岛素及胰岛素抵抗指数(HOMA-IR)变化的影响。方法给予56名健康青年7d平衡膳食和6d高糖膳食。平衡膳食的热量组成为15%蛋白质、31%脂肪和54%碳水化合物;高糖膳食的热量组成为15%蛋白质、15%脂肪和70%碳水化合物。于膳食干预的第1d、第8d和第14d清晨抽取12h空腹静脉血,测定血脂、血糖及胰岛素浓度,计算HOMA-IR。提取全基因组DNA,聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP)分析apoA1+83C/T多态性。结果高糖膳食后甘油三酯(TG)(P=0.000)和胰岛素(P=0.023)仅在CC基因型受试者中升高。膳食后总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)在CC基因型受试者和T携带者中均降低(P<0.05),高密度脂蛋白胆固醇(HDL-C)在CC基因型受试者(P=0.048)和T携带者(P=0.028)中均升高。结论高糖膳食诱导的TG及胰岛素变化受apoA1+83C/T多态性的调节,T等位基因可以抵消高糖膳食引起的TG和胰岛素升高。

Objective The aim of the present study was to investigate the possible effects of the ＋83C/T polymorphism in apolipoprotein AI gene（apoA1） on the changes of serum lipids,glucose,insulin and the homeostasis model assessment of insulin resistance（HOMA-IR） induced by a high-carbohydrate diet in healthyyouth.Methods Fifty-six participants were given a washout diet for 7 d,followed by a high-carbohydrate diet for 6 d.The washout diet contained 15% protein,31% fat and 54% carbohydrate.The high-carbohydrate diet contained 15% protein,15% fat and 70% carbohydrate.Twelve hour fasting venous blood was drawn on the mornings of the first,eighth and fourteenth days.Blood lipids,glucose and insulin were measured,and HOMA-IR was calculated.The genome DNA was extracted and the apoA1 ＋83C/T polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism.Results Triglyceride and insulin were found significantly increased in the subjects with the CC genotype,but not in the T carriers after the high-carbohydrate diet.Significant decreases of total cholesterol and LDL-C and a significant increase of HDL-C were observed after the dietary intervention of the high-carbohydrate diet.Conclusion The triglyceride and insulin changes after the high-carbohydrate diet can be modulated by the apoA1 ＋83C/T polymorphism,and the T allele may eliminate the increase in triglyceride and insulin induced by the high-carbohydrate diet.