栀子和善宁联合应用对重症急性胰腺炎大鼠线粒体损伤的影响

Effects of the Combination of Sandostatin and Gardenia Jasminoides Ellis on Pancreatic Mitochondria Injury in Severe Acute Pancreatitis Rats

目的探讨栀子和善宁联合应用对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠线粒体损伤的保护作用。方法经胆胰管逆行注射15g/L去氧胆酸钠建立SAP模型,随机分为5组(n=8):胰腺炎组、对照组、善宁组、栀子组、联合组,观察胰腺线粒体超氧化物歧化酶(SOD)、琥珀酸脱氢酶(SDH)、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性,丙二醛(MDA)含量以及膜流动性的变化,并进行相关分析。结果与对照组比较,胰腺炎组胰腺线粒体SOD、SDH、Na+-K+-ATP酶活性和Ca2+-Mg2+-ATP酶的活性降低,MDA含量以及膜微黏度增高(P<0.01),同时伴有血中淀粉酶升高(P<0.01);栀子组、善宁组及联合组上述各检测指标均较胰腺炎组明显改善(P<0.01),但以联合组作用明显(P<0.05或P<0.01);相关分析显示,MDA与SOD、膜流动性、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性间呈负相关(r=-0.857,P<0.01;r=-0.960,P<0.01;r=-0.966,P<0.01;r=-0.926,P<0.01)。结论栀子和善宁联合应用对SAP大鼠线粒体损伤具有保护作用,疗效优于单一的西药或中药治疗。

Objective To study the protective effects of the combination of sandostatin（SS） and gardenia jasminoides ellis（GJE） on pancreatic mitochondria injury in severe acute pancreatitis（SAP） rats.Methods SAP rat model was induced by retrograde injection of 15 g/L sodium deoxycholate into biliary tract.The rats were randomly divided into five groups：SAP group,Sham group,SS group,GJE group,as well as SS and GJE combination group.The changes of Superoxide dismutase（SOD）,succinic dehydrogenase（SDH）,Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase,malondialdehyde（MDA）,membrance fluidity of mitochondria in pancreatic tissue were measured.Results Compared to Sham group,the activities of SOD,SDH,Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in mitochondria were obviously decreased in SAP group,and MDA and microviscosity（η） were markedly increased（P〈0.01）,which were associated with the increase of serum amylase level（P〈0.01）.The above-mentioned indexes of GJE group,SS group and combination group were obviously ameliorated in comparison with SAP group（P〈0.01）,the improvement in combination group was the best（P〈0.05 or P〈0.01）.The correlation analysis indicated that MDA was negatively correlated with SOD,membrance fluidity,as well as the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase（r=-0.857,P〈0.01;r=-0.960,P〈0.01;r=-0.966,P〈0.01;r=-0.926,P〈0.01）.Conclusion The combination of sandostatin and gardenia jasminoides ellis can protect pancreatic mitochondria injury in severe acute pancreatitis.