胡杨SCL7基因及其启动子片段的克隆与分析

Cloning and analysis of SCL7 gene from Populus euphratica

根据拟南芥SCL7基因序列,以杨树基因组数据库为背景克隆得到了胡杨胁迫诱导基因SCL7全长及其启动子片段,所得基因命名为PeSCL7。序列分析表明:该基因的开放阅读框1767bp,编码588个氨基酸,该基因不含内含子。同源性比对发现该基因属于GRAS/SCL蛋白家族中SCL4/7分支;半定量RT-PCR结果显示,PeSCL7的表达受到多种逆境胁迫的诱导,且当胁迫处理3h时表达量达到最大值;对器官表达特异性进行分析,发现PeSCL7在胡杨的根、茎、叶中均有表达。克隆得到的胡杨PeSCL7启动子序列长968bp,包含可能与干旱胁迫、病害胁迫等应答元件。

To characterize the function of SCL7 in Populus euphratica, we cloned the full-length PeSCL7 gene and promoter region based on the AtSCL7 sequence of Arabidopsis and the Populus database. DNA sequence analysis showed that the PeSCL7 gene contained an open reading frame of 1 767 bp long without intron and encoded a 588 amino acid polypeptide. Similarity analysis showed that it belonged to the SCL4/7 branch of GRAS/SCL family. RT-PCR analysis revealed that the PeSCL7 could be induced by various stresses and the expression reached a maximum after 3 hrs of induction. PeSCL7 was detected in leaves, stems and roots in P. euphratiea via the analysis on the organ-specific expression. The PeSCL7 promoter sequence, about 968 bp in length, contained many stress-responsive cis-acting elements, i. e. , ABRE, MYB and WRKY core sequences. The results indicate that the PeSCL7 gene may play a significant role in stress responses of P. euphratica.