日本落叶松无性系扦插生根过程中多酚类物质研究

Qualitative analysis of phenolic compounds in the Japanese larch during the rooting of cuttings

对4个日本落叶松优良无性系(日抚27-3-1、日宽17-3-1、日草82-4-3、日草103-3-1)扦插生根过程中插穗生根性状和插穗内源多酚类物质含量进行了动态分析。结果表明:日本落叶松无性系扦插生根性状存在较大差异,日抚27-3-1和日宽17-3-1较易生根,日草103-3-1和日草82-4-3难生根。插穗内源多酚类物质变化趋势差异较大,总酚酸含量随扦插时间的延长而不断降低,且较易生根的2个无性系插穗内的各种酚类物质含量均低于难生根的2个无性系;其中日抚27-3-1和日宽17-3-1插穗内邻苯二酚、对羟基苯甲酸、阿魏酸、儿茶酸和没食子酸含量都随扦插时间而不断减小,且减小幅度较小;日草82-4-3、日草103-3-1插穗内邻苯二酚、阿魏酸、儿茶酸和没食子酸减小幅度较大,且波动性较大,相反,邻苯二酚含量却呈升高趋势。无性系插穗的生根率、生根量、最长根长度与插穗内各种酚类物质含量总体呈负相关关系,但不同酚类物质对插穗生根所产生的抑制作用不同。

The changes of rooting characteristics and contents of endogenous phenolic compounds were studied during the rooting period of cuttings of four elite Japanese larch （Larix kaempferi） clones, i. e. , Rifu27- 3-1, Rikuan 17-3-1, Ricao824-3, and Ricao103-3-1. Results showed that there were significant differences in the rooting characteristics of cuttings amongst different clones. Clones ＂Rifu27-3-1＂ and ＂Rikuan17-3-1＂ were easier to root than the other two clones. Marked differences were found in the variation of contents of endogenous phenolic compounds during the rooting period for the clones, but, in general, the total content of phenolic compounds decreased over the rooting period. The contents of phenolic compounds in two easy-to-root clones were lower than those of the two difficult to root. The contents of pyrocatechol, para hydroxyl-benzoic acid, ferulic acid, pyrocatechuic acid and gallic acid in clones ＂Rifu27-3-1＂ and ＂ Rikuan 17-3-1 ＂ decreased slightly during the rooting of cuttings, while the contents of pyrocatechol, ferulic acid, pyrocatechuic acid and gallic acid in clones ＂Ricao82-4-3＂ and ＂Ricao103-3-1＂ declined sharply with great fluctuation except for pyrocatechol which increased on the contrary. Generally, a significantly negative relationship existed between the contents of phenolic compounds and variables like rooting rate, number of roots and maximum root length. Restraining effects of phenolic compounds on rooting of cuttings varied.