摘要
目的研究静脉给予的外源性神经干细胞在淀粉样前体蛋白(APP)小鼠脑内的分布及可能起到的作用。方法采用14d小鼠胚胎的神经干细胞,经体外扩增、分化后,静脉注射到阿尔茨海默病动物模型——APP转基因小鼠体内,1周后通过磁共振成像(MRI)及激光共聚焦显微镜观察APP小鼠脑内外源性移植神经干细胞的分布。结果外源性标记的神经干细胞可通过血-脑屏障进入APP小鼠脑内,聚集在β-淀粉样蛋白(Aβ)沉积周围,部分外源性细胞内出现Aβ信号。结论外源性神经干细胞可能在病变区神经元修复或Aβ清除方面起到一定的作用。
Objective To study the distribution and possible effects of exogenously administered neural stem cells in amyloid precursor protein（APP） mouse brain.Methods Neural stem cells from 14-day embryo were administered into APP transgenic mice tail veins after amplification and differentiation in vitro.One week later,the distribution of the exogenous neural stem cells was observed by magnetic resonance imaging（MRI） and laser scanning confocal microscope.Results Exogenous neural stem cells passed through the blood-brain barrier and accumulated around β-amyloid（Aβ） deposits in APP mouse brain.Some Aβ signals were detected in exogenous cells.Conclusion It is demonstrated that exogenous neural stem cells play a potential role in neuron repairing or Aβ clearing in lesion of APP mouse brain.
出处
《生物医学工程与临床》
CAS
2011年第1期21-23,F0002,共4页
Biomedical Engineering and Clinical Medicine
基金
辽宁省教育厅科研项目(2008738)
辽宁省教育厅科研项目(L2010579)
