RNAi沉默livin基因对裸鼠移植瘤生长及Ki67蛋白表达的影响

Effects of silencing livin gene by RNA interference on transplanted tumor growth and Ki67 expression in nude mice

目的利用慢病毒携带的livin基因短发夹RNA(shRNA)干扰裸鼠移植瘤中Livin蛋白表达,探讨其对肺腺癌裸鼠移植瘤生长及Ki67蛋白表达的影响。方法建立肺腺癌裸鼠皮下移植瘤模型,待移植瘤长至100mm3左右时,以携带livin基因shRNA慢病毒载体注入移植瘤,观察肿瘤生长情况。用免疫组织化学方法检测各组Livin蛋白、Ki67蛋白表达,分析二者相关性。结果 livin基因shRNA干预组与空白对照组和阴性载体对照组相比,瘤体生长减缓,移植瘤质量明显减小(P<0.01),Livin蛋白、Ki67蛋白的表达明显降低(P<0.01,P<0.01),且Livin蛋白与Ki67蛋白的表达呈正相关(r=0.54,P<0.01)。结论利用livin基因shRNA可抑制移植瘤生长,Livin蛋白表达明显降低,Ki67蛋白的表达也降低,两者呈显著正相关。

Objective To inhibit the livin gene expression in nude mice by lentiviral vector carrying livin shRNA,and observe its effect on transplanted tumor growth and Ki67 expression.Methods SPC-A1 was injected into nude mice to form subcutaneous lung adenocarcinoma model.Lentiviral vector with livin shRNA was injected into tumor to induce RNAi when the volume of tumor reached 100 mm3,meanwhile the tumor growth was observed.The expression of Livin and Ki67 protein were detected by immunochemistry to analyse the correlation of the livin and ki67 gene expression.Results The study showed a slower tumor growth and a lighter tumor weight in experimental group than the blank and negative control groups.Livin and Ki67 protein expression level were significantly lower than the blank group and the negative group,and the 2 proteins had positive correlation.Conclusion RNA interference targeting livin inhibits tumor growth and decreases the expression of Livin and Ki67 protein.At the same time the 2 proteins have a significantly positive correlation.