鲤回交群体4种生长性状的相关性分析

Correlation analysis of the 4 growth traits in the common carp backcross population

利用132对SSRs(simple sequence repeat)标记和63对在EST(expressed sequence tag)上的微卫星标记对柏氏鲤和荷包红鲤抗寒品系回交子代的84个个体基因组DNA进行检测,共得到565个等位基因,各等位基因数2～4个,片段长度72～484 bp,平均等位基因数为2.897 4,平均多态信息含量为0.584 9,平均杂合度为0.592 8。利用统计软件SPSS的GLM程序对195个微卫星标记与鲤体重、体长、头长和尾柄长的相关性进行分析,其中HLJ133、HLJ346、HLJ360与体重具有极显著相关;HLJ133,HLJ368,HLJ390,HLJ673,HLJE96与体长具有显著相关;HLJ133,HLJ346,HLJ360与头长极显著相关;HLJ529,HLJE538,HLJE586与头长具有显著相关;HLJ673,HLJE282与尾柄长具有极显著相关;HLJ348,HLJ368,HLJ382,HLJ437,HLJ483,HLJ752,HLJE169,HLJE586与尾柄长具有显著相关,并对同一标记不同基因型间进行了多重比较。根据实验求出的体重与体长、头长、尾柄长之间的相关系数和通径系数,可得知体重与尾柄长的直接相关性非常小。

132 SSR and 63 EST markers were used to analyze the genotype of eighty-four individuals which crossed from barbless carp（Cyprinus pellegrini） and Hebao cold tolerance red carp（Cyprinus carpio var.wuyuanensis）.The results showed that a total of 565 different alleles were found,and the number of alleles in each locus was 2 to 4.The DNA fragment length was 72 bp to 784 bp,and the average number of alleles per locus was 2.897 4.The mean polymorphism information content（PIC） was 0.584 9,and the average heterozygosity was 0.592 8.The GLM procedure was used to analyze the effects of these 195 microsatellites on body weight,length,head length and caudal peduncle length.Results revealed that HLJ133,HLJ346,HLJ360 were discovered very significantly related to carp’s body weight,HLJ133,HLJ368,HLJ390,HLJ673,HLJE96 had a significant impact on carp’s body length.Markers HLJ133,HLJ346,HLJ360 were discovered very significantly related to carp ’s head length,and HLJ529,HLJE538,HLJE586 had a significant impact on carp’s head length.HLJ673,HLJE282 were discovered very significantly related to carp’s caudal peduncle length,and HLJ348,HLJ368,HLJ382,HLJ437,HLJ483,HLJ752,HLJE169,HLJE586 had a significant impact on carp’s caudal peduncle length.In addition,the correlation coefficient and the path coefficient were obtained between length,head length,caudal peduncle length and body weight.And the correlation between caudal peduncle length and body weight was insignificant.