角膜缘干细胞的识别与分离研究进展

Research advances on identification and purification of limbal stem cells

角膜缘干细胞（LSCs）是角膜上皮组织维持自我更新和损伤后修复的基础，对角膜的完整性和透明性至关重要。对LSCs的准确识别与分离有助于深入探讨LSCs的性质及用于治疗LSCs缺乏。目前发现的LSCs分子标志物的数量较多，但其特异性均存在差异，多种利用干细胞属性间接标记LSCs的方法有助于对LSCs的分离和识别。就近年来利用分子生物学标记、细胞形态及功能在识别LSCs方面的研究进展，以及纯化分离LSCs的新技术进行综述。

Limbal stem cells （LSCs） are essential for the integrity and transparency of the cornea,because they provide new epithelial ceils whatever physiological state and wound healing condition. Specific characterization and isolation of LSCs will greatly contribute to a deep exploration about their biological nature and application in the treatment of LSCs deficiency. Since varieties of molecular markers and general properties of LSCs have been identified so far and are diverse in their specificity for LSCs,it is important for us to learn the unique characteristics of each and further to understand and manipulate them in a more efficient way. This article aimed to review the identification of LSCs based on molecular marker and general properties of limbal stem cells and summarize the techniques for purifying these cells.