心房颤动与白介素-6-174G/C、-572C/G、-597G/A位点基因多态性的关系

Relationship between Atrial Fibrillation and the IL-6-174G/C,-572C/G and -597G/A Polymorphism

目的:探讨江苏南通地区汉族人群中白细胞介素(IL)-6-174G/C(rs1800795)、-572C/G(rs1800796)、-597G/A(rs1800797)单核苷酸多态性(SNP)与血清IL-6水平以及房颤发病之间的关系。方法:分别测定房颤组和对照组血清IL-6及其他临床指标,测量左心房长径(LAD);提取基因组DNA,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术扩增目的基因片段并酶切。结果:房颤组IL-6和LAD与对照组比较差异有统计学意义(P﹤0.01);房颤组IL-6-572C/G基因型分布G/C+G/G明显高于对照组(P﹤0.05),且房颤组G等位基因频率明显大于对照组(P﹤0.01),IL-6-174G/C位点C等位基因突变率极低,IL-6-597G/A未见A等位基因突变。结论:IL-6-572C/G位点G等位基因突变导致了机体血清IL-6水平升高,可能与房颤的发病有关。

Objective： To explore the relationships among atrial fibrillation （AF）, interleukin-6 （IL-6） and single nucleotide polymorphisms （SNP） of the IL-6-174G/C, -572C/G and -597G/A. Methods： The serum levels of IL-6, lipid and other clinical indicators were measured. The left atrial diameter （LAD） was measured by echocardiography （UCG）. The frequency distributions of genotypes and alleles of the IL-6-174G/C, -572G/C and -597G/A were amplified by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in samples of 127 AF patients. Results： The values of IL-6 and LAD were significantly higher in AF group than those of control group （P 0.01）. The G/C＋G/G genotype distribution and G allele frequencies of IL-6-572C/G were significantly higher in AF group than those of control group （P 0.01）. The C allele mutation rate of IL-6-174G/C was minimal in Han population in Jiangsu Nantong, and no A allele mutation in IL-6-597G/A. Conclusion： The results showed that among Han population in Nantong IL-6-572 C/G polymorphic allele G mutation may lead to the increase of serum level of IL-6 in AF group,which may be related to the pathogenesis of AF.