阿片生长因子抑制乳腺癌细胞生长的研究

Inhibitory Effect of Opioid Growth Factor on Human Breast Carcinoma Cells

目的:探讨阿片生长因子(OGF)对MCF-7人乳腺癌细胞生长的影响。方法:细胞计数观察OGF、阿片生长因子受体(OGFr)拮抗剂纳洛酮(Naloxone)组及对照组细胞数的差别;MTT实验观察应用OGF、Naloxone及OGF联合Naloxone(OGF+Naloxone)细胞增殖抑制率的变化;流式细胞术(FCM)细胞周期分析OGF、Naloxone对MCF-7细胞生长的影响。结果:细胞计数显示,OGF处理组细胞数明显低于Naloxone处理组及对照组(P<0.01),而后2组细胞数差异无统计学意义(P>0.05);MTT实验显示,OGF处理组细胞增殖抑制率明显高于Naloxone处理组及OGF+Naloxone处理组(P<0.01),而后2组差异无统计学意义(P>0.05);OGF处理组MCF-7细胞G0/G1期分数明显高于Naloxone处理组及对照组(P<0.01),而后2组差异无统计学意义(P>0.05)。结论:OGF能抑制MCF-7人乳腺癌细胞的生长。OGFr拮抗剂能阻断这种作用。OGF阻断细胞周期于G0/G1期。

Objective：To investigate the inhibitory effect of opioid growth factor （OGF）on MCF-7 human breast carcinoma cells. Methods：The difference in cell counting was compared between OGF group and the opioid antagonist naloxone group. The MTT [3-（4,5-Dimethylthiazol-2-yl）-2,5-Diphenyltetrazolium Bromide] assay was used to detect the inhibiting rate of cell growth in OGF, naloxone and OGF combined naloxone（OGF＋naloxone）groups. And flow cytometry was used to detect the effects of OGF and naloxone on the cell cycle distribution in MCF-7 cells. Results： The results of cell counting showed that the cell concentration reduced markedly in OGF group than those of naloxone and control groups （P 0.01）. No difference was found between naloxone and control groups （P 0.05）. MTT assay indicated that the inhibiting rate of the cell growth was greatly higher in OGF group than those in naloxone and OGF＋ naloxone groups （P 0.01）, but there was no significant difference between the latter two groups （P 0.05）. Flow cytometry results showed that cell fraction was lower in OGF group than those in naloxone and control groups （P 0.01）, and no significant difference was found between the latter two groups （P 0.05）.Conclusion： OGF can inhibit the growth of MCF-7 cells in vitro, and this inhibitory effect can be blocked by opioid antagonist naloxone. OGF can induce the cell cycle arrest at G0/G1 phase.