鸭Akirin2基因克隆、序列分析与组织表达特性研究

Cloning,Sequence Analysis and Specific Expression in Different Tissues of Duck Akirin2 Gene

为研究Akirin2基因在鸭生物体中的功能,本试验通过RT-PCR扩增了鸭Akirin2基因,并采用荧光定量PCR方法检测了其在40日龄北京鸭不同组织中的表达差异。序列分析结果表明,鸭Akirin2基因CDS区全长594bp,编码197个氨基酸,氨基酸水平上与鸡的相似性达99%,其氨基酸序列二级结构具有哺乳动物Akirin1和Akirin2氨基酸二级结构域的共同区域;荧光定量结果表明,鸭Akirin2在肌肉和免疫器官脾脏中都有表达。以上结果支持了鸟类Akirin2可能具有与哺乳动物中Akirin1和Akirin2共同功能的观点,为进一步研究Akirin2基因在鸟类肌肉发育中的作用奠定了基础。

In order to understand the function of Akirin2 gene in duck,the duck Akirin2 gene was cloned by RT-PCR,and the relative mRNA expression of Akirin2 was detected in different tissues using the qPCR method.The results of the sequence analysis showed that the CDS region of the duck Akirin2 gene included 594 nucleotides,encoding for 197 amino acids,and the amino acid homology of Akirin2 between the duck and the chicken was 99%.The secondary structure of Akirin2 amino acids had a common domain with the mammals Akirin1 and Akirin2.The results of the qRT-PCR indicated that the duck Akirin2 express in the muscle and the immune organ spleen.These results supported the previously views that duck Akirin2 gene had a common function with the Akirin1 and Akirin2 genes in mammals,and these assays made a foundation work for the further research about the Akirin2 gene in myogenesis in birds.