乙酰甲喹主要代谢物在鸡粪的排泄量研究

Studies on the Determination of Main Metabolites of Mequindox Excretion in Chicken Feces

本研究旨在建立鸡粪便中乙酰甲喹代谢物M1、M2的高效液相色谱检测方法,测定乙酰甲喹主要代谢物在鸡粪的排泄量。对15只7周龄健康科宝-500白羽鸡内服乙酰甲喹30mg.kg-1体质量后,于不同时间间隔收集其所有粪便,称重,取其中2g用体积比1︰1的二氯甲烷/乙腈混合液提取,吹干,残渣用30%甲醇/水溶液复溶,HPLC法检测。结果表明,代谢物M1、M2检测限与定量限分别为0.01、0.05μg.mL-1,代谢物M1、M2添加浓度在0.05、1.0、50μg.mL-1时,平均回收率范围分别为69.5%～72.4%、73.5%～80.6%。粪便中代谢物M1、M2占给药乙酰甲喹总量的比例分别为3.20%、4.58%。代谢物M1排出高峰期在给药后4～8h,占代谢物M1累积排泄总量的38.09%;代谢物M2排出高峰期在12～24h,占代谢物M2累积排出总量的42.29%。结果提示,该检测方法满足测定乙酰甲喹主要代谢物在鸡粪中排泄量的要求。

In order to investigate the excretion quantitation of main metabolites of mequindox in chicken feces,a high performance liquid chromatography method was developed to detect the concentrations of metabolites M1,M2 of mequindox.Fifteen seven-week-old chickens were administered mequindox orally at a dose of 30 mg·kg-1 BW.After administration,all of their feces were collected at different time intervals and weighed.Then 2 g of feces were extracted using methylene chloride/acetonitrile（1︰1 V/V） solution and the organic phase was separated and evaporated.The residual was redissolved with methanol/water（30︰70 V/V） solution,and then analyzed by HPLC.The results showed that the limit of detection（LOD） and limit of quantitation（LOQ） of metabolites M1 and M2 were 0.01 and 0.05 μg·mL-1,respectively.The range of average recovery of metabolites M1 and M2 were 69.5%-72.4%,73.5%-80.6% under the concentrations of 0.05,1.0 and 50 μg·mL-1.The proportions of metabolites M1 and M2 in total amount of mequindox administered were 3.20% and 4.58%,respectively.The excretion peak time of metabolite M1 was 4-8 h with 38.09% of accumulated excretion total amount of metabolite M1.And the excretion peak time of metabolite M2 was 12-24 h with 42.29% of accumulated excretion total amount of metabolite M2 after administration.The present results indicated that the method developed in this study could be used to detect the concentration of metabolites M1 and M2 of mequindox in chicken feces.