摘要
为了阐明Ghrelin对大鼠下丘脑与垂体的调控规律,拟采用PV-9000两步法免疫组化和原位杂交方法分别确定Ghrelin及其mRNA在大鼠下丘脑和垂体组织中的定位。结果表明Ghrelin及其mRNA主要分布在下丘脑的弓状核、腹内侧核、正中隆起、室旁核等处的核团,各部分核团细胞着色程度深浅不一,腺垂体细胞中也有大量阳性细胞分布,但神经垂体则无阳性细胞分布。结果提示,Ghrelin及其mRNA在大鼠下丘脑、垂体中均有分布,揭示Ghrelin可能对大鼠促性腺激素释放激素和促性腺激素分泌有一定的调控作用。
To investigate the regulation of Ghrelin on the hypothalamus and pituitary of rat.The localization of Ghrelin and its mRNA in hypothalamus and pituitary tissues were detected by immunohistochemical PV-9000 2-step and in situ hybridization,respectively.Results showed that Ghrelin and its mRNA was mainly distributed in the arcuate nuclei,ventromedial nucleus,median eminence,periventricular nucleus of hypothalamus and anterior pituitary,the colour of cells in nuclei were different.A large number of positive cells were found in anterior pituitary,no positive cells were distributed in neurohypophysis.Ghrelin and its mRNA were both presented in the hypothalamus and pituitary tissues of rat,which suggested that the Ghrelin may play a role in the secretion of gonadotropin-releasing hormone and gonadotropin.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2011年第1期131-135,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
安徽省自然科学基金项目(070411015)