大蒜油及大鼠周龄和性别对正己烷体内代谢的影响

Effects of garlic oil, age and sex on n-hexane metabolism in rats

目的探讨大蒜油及周龄、性别对正己烷在大鼠体内代谢的影响。方法以Wistar大鼠为实验动物，（1）灌胃染毒：正己烷组（3000mg／kg正己烷），大蒜油干预组（染毒前1h予80mg／kg大蒜油灌胃），8、12、16、20、24、28、32h断尾取血。（2）腹腔注射染毒：正己烷组（1000mg／kg正己烷），大蒜油干预组（染毒药前1h予80mg／kg大蒜油灌胃），8、12、16、20、24、28h断尾取血。（3）6、8、10周龄（均为7只）雄性大鼠予正己烷3000mg／kg灌胃，染毒后16、20、24h断尾取血。（4）8周龄大鼠雌、雄各7只，予正己烷3000mg／kg灌胃，染毒后16、20、24、28h断尾取血。气相色谱法测定血清中2，5-己二酮浓度，比较给和不给大蒜油、不同周龄、不同性别大鼠血清中2，5-己二酮浓度。结果（1）灌胃染毒：正己烷组、大蒜油干预组血清中2，5-己二酮浓度分别在20和24h达到峰值，分别为19．2和12．3μg／ml，在峰值及之前时间点大蒜油干预组血清中2，5-己二酮浓度明显降低，但峰值后的消除过程明显减缓。（2）腹腔注射染毒：大蒜油对血清中2，5-己二酮浓度的影响与正己烷灌胃途径基本相同，两组分别在12和16h达到峰值，分别为15．0和6．7μg／ml。（3）周龄：16h时6、8、10周龄大鼠血清中2，5-己二酮浓度分别为25．5、15．0、12．8μg／ml，8、10周龄与6周龄的差异有统计学意义（P〈O．05或P〈0．01）；20h时6、8、10周龄大鼠血清中2，5-己二酮浓度分别为24．7、18.3、15．0μg／ml，10周龄与6周龄的差异有统计学意义（P〈O．05）；24h时6、8、10周龄大鼠血清中2，5-己二酮浓度分别为11．0、14．7、8．1μg／ml，10周龄与8周龄的差异有统计学意义（P〈0．05）。（4）性别：16h时雄性、雌性大鼠血清中2，5-己二酮浓度分别为22．5、17．2μg／ml，差异有统计学意义（P〈O．05）；在20、24、28h雄性、雌性大鼠血清中2，5-己二酮浓度分别为27．6、22．9μg／ml，24．6、19．1μg／ml，19．1、13．8μg／ml，不同性别间的差异均无统计学意义（尸〉0．05）。结论大蒜油可明显减少正己烷在大鼠体内代谢物2，5-己二酮的生成量；低周龄的动物正己烷的代谢能力高于高周龄的动物。

Objective To investigate effects of garlic oil （GO）,age and sex on n-hexane metabolism in rats. Methods The Wistar rats were used as experimental animals. （1） Intragastric administration： nhexane group （3000 mg/kg n-hexane）,GO treated group （80 mg/kg GO ig. an hour earlier than 3000 mg/kg n-hexane）, then blood was taken from tails of rats at 8, 12, 16, 20, 24, 28, 32 h points after n-hexane administration. （2）Intraperitoneal injection： n-hexane group （1000 mg/kg n-hexane）, GO treated group（80 mg/kg GO ig. an hour earlier than 1000 mg/kg n-hexane）, then took blood was taken from tails of rats at 8, 12, 16, 20, 24, 28 h points after n-hexane injection. （3） 7 rats each group of 6, 8, 10 weeks age were administrated by 3000 mg/kg n-hexane intragastrieally, then were taken blood from tails at 16, 20, 24 h points after administration.（4） 7 male and 7 female rats of 8 weeks age were administrated by 3000 mg/kg n-hexane intragastrieally, then were taken blood from tails at 16, 20, 24, 28 h points after administration. The gas chromatography was used to determine the metabolite 2, 5-hexanedione concentration of n-hexane in serum and 2, 5-hexanedione concentration was compared between GO and no GO treated rats, different ages and differ- ent sexes. Results （1）Intragastric administration： 2, 5-hexanedione concentrations in serum of n-hexanegroup and GO treated group had the peak 19.2 and 12.3 μg/ml at 20 h and 24 h points. Compared with nhexane group, the serum 2, 5-hexanedione concentration of GO treated group was lower at time points prior to peak and 2, 5-hexanedione eliminating process was slower after peak.（2） Intraperitoneal injection： effects of GO on the serum 2, 5-hexanedione concentrations was very similar to intragastric administration, 2, 5- hexanediane concentrations in serum of n-hexane group and GO treated group had the peak 15.0 and 6.7 μg/m] at 12 h and 16 h points. （3） Comparison of the serum 2, 5-hexancdione concentrations of different weeks age rats： The serum 2, 5-hexancdione concentrations of 6, 8, 10 weeks age rats were 25.5, 15.0, 12.8 μg/ml each （8, 10 weeks age significantly lower than 6 weeks age） at 16 h point; at 20 h point, they were 24.7, 18.3, 15.0 μg/ml each （10 weeks age significantly lower than 6 weeks age）; at 24 h point, they were 11.0, 14.7, 8.1 μg,/ml each （10 weeks age significantly lower than 8 weeks age）. （4） Comparisons of the serum 2, 5-bexanedione concentrations of different sex rats： the serum 2, 5-hexanedione concentrations of male and female rats were 22.5, 17.2 μg/ml each at 16 h point （different significantly）; at 20, 24, 28 h points, they were 27.6, 22.9 μg/ml, 24.6, 19.1 μg/ml, 19.1, 13.8 μg/ml each （different non-significantly）. Conclusion GO reduces production of 2, 5-hexanedione in serum generated by n-hexanc in rats; the metabolic capacity of low age rats on n-hcxane is stromger than high age ones.