中国沿海地区汉族男性人群白细胞介素-1β-31C/T基因多态性与痛风的易感性研究

Relationship between interleukin-1β -31C/T polymorphism and susceptibility to gout in Chinese male population

目的 探讨山东省沿海地区中国汉族男性群体的白细胞介素（IL）-1β启动子区rs1143627（-31C/T）基因多态性的分布状况及其与痛风易感性之间的关系.方法 选取208例痛风患者和210名健康对照者,应用聚合酶链反应限制性片段长度多态性（RFLP）技术,检测中国汉族男性群体的IL-1β启动子区rs1143627（-31C/T）位点基因多态性与痛风发病的遗传易感性的关系.采用Hardy-Weinberg检验确认标本的群体代表性,数据分析采用χ2检验和t检验.结果 痛风组中IL-1β启动子区-31C/T位点CC,CT和TT基因型分别为32.7%,43.3%和24.0%,健康对照组分别为31.9%,46.2%和21.9%,2组比较差异无统计学意义（χ2=0.427,P〉0.05）;2组的等位基因频率C和T间差异也无统计学意义（分别为54.3%,55.0%;45.7%,45.0%;χ2=0.038,P〉0.05）.经χ2检验,IL-1β基因-31C/T位点基因多态性与痛风病的危险因素无显著性关联.结论 尚不能认为中国沿海地区汉族男性人群中IL-1β启动子区rs1143627（-31C/T）基因多态性与痛风有关联性.

Objective To explore gene polymorphism of the C/T genotype of rs1143627 in the promoter of IL-1β gene in male population living in the coastal area of Shandong, and thus to investigate the relationship between the gene polymorphism of IL-1β and gout. Methods A total of 208 gout patients and 210 healthy controls were enrolled. The possible association between the polymorphism of IL-1 β -3 1C/T and gout in Chinese were investigated and genotype frequencies and allelic frequencies was calculated by polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） method. Hardy-Weinberg was used to verify the representativeness of the sample. Comparisons between the groups were performed with χ2 test and t-test. Results The frequencies of CC, CT, and TT genotypes were 32.7%, 43.3% and 24.0%,respectively among gout patients, while they were 31.9%,46.2% and 21.9%, respectively among the controls.There was no statistically difference in IL-1β -31C/T genotype frequencies between gout patients and controls （χ2=0.427, P〉0.05）. The allele frequencies of C and T in gout cases were different from those in the controls （54.3%, 55.0%; 45.7%, 45.0%; χ2=0.038, P〉0.05）. Moreover, no association between IL- I β-31 C/T genotypes and risk factors for gout were observed in gout cases by χ2 test. Conclusion Results of the present study suggest that the C/T genotype of rs1143627 in the promoter of IL-1β gene is not associated with gout in male population living in the coastal area of Shandong.