簇生沿丝伞液体发酵培养及其发酵物体内抗肿瘤活性研究

Fermentation culture of Naematoloma fasciculare and antitumor activities of its fermented broth in vivo

采用中心组合旋转设计法确定了簇生沿丝伞液体深层培养菌丝体的最佳方案。采用H22荷瘤小鼠进行体内抗肿瘤试验,对簇生沿丝伞发酵物的抗肿瘤活性进行了探究。结果表明,当培养基配方为麦芽糖59.27g/L,蛋白胨:酵母粉=1:1为8.04g/L,磷酸二氢钾:硫酸镁:硫酸铵=1:1:1为2.27g/L时,生物量达到最高,为15.06g/L。簇生沿丝伞发酵物的抑瘤率与剂量存在明显的量效关系,200mg/kg时,抑瘤率达最高,为54.71%,且白细胞数和IL-2的含量与对照组相比均显著增高。

Optimum conditions of fermentation culture of Naematoloma fasciculare were investigated by using central composite rotatable design. Antitumor activities of fermented broth of N. fasciculare were observed on H22 implanted mice in vivo. The results showed that the maximal biomass was 15.06g/L when the composition of the medium was 59.27g/L maltose,8.04g/L peptone：yeast powder=1：1 and 2.27g/L KH2PO4：MgSO4：（NH4）2SO4=1：1：1. There was an obvious dose-effect relationship between dosages of fermented broth of N. fasciculare and antitumor rates,and the maximum inhibition rate reached 54.71% at concentration of 200mg/kg. In addition,WBC （109/L） and content of IL-2 of the mice in the group at concentration of 200mg/kg were all improved obviously as compared with control group.