金匮肾气丸联合葡萄糖酸钙对去势大鼠骨质疏松的影响

Effect of combined use of Jingui shenqi wan and calcium gluconate on ovariectomy-induced osteoporosis in rats

目的观察金匮肾气丸联合葡萄糖酸钙对去卵巢大鼠骨质疏松的影响,探究其防治骨质疏松的机制。方法将75只雌性大鼠随机分成空白对照组,模型组,葡萄糖酸钙组,金匮肾气丸组,金匮肾气丸及葡萄糖酸钙联合用药组共5组,采用卵巢切除的方法制成骨质疏松动物模型,灌胃给药3个月后进行血钙,磷,骨钙含量的测定和组织病理学的观察。结果模型组与空白对照组相比,去势大鼠血清钙磷含量无明显变化,钙片组、金匮肾气丸组及联合组血钙,磷含量相比空白组也无明显差异;去势大鼠骨钙含量较正常大鼠明显降低,钙片组,金匮肾气丸,以及联合组去势大鼠骨钙含量较模型组明显改善,以联合组改善最明显(P<0.01)。组织病理学观察见:经钙片和金匮肾气丸治疗后骨皮质可见蚕食样改变区域变小,骨小梁较细,排列较为一致。联合用药组骨皮质厚度基本正常,骨小梁略变细,排列较整齐,骨小梁厚度基本正常。结论金匮肾气丸联合葡萄糖酸钙可以减少去卵巢骨质疏松大鼠骨矿物质的丢失,对骨质疏松具有较好的防治作用。

Objective To investigate the effect of combined use of Jingui shenqi wan and calcium gluconate on ovariectomy-induced osteoporosis in rats, and to explore the mechanism of prevention and treatment of osteoporosis. Methods Seventy five female rats were randomly divided into control group, model group, calcium gluconate group, Jingui shenqi wan group, and Jingui shenqi wan plus calcium gluconate group. Ovariectomy was performed to induce osteoporosis animal model. Drugs were intragastricly administered for 3 months. Serum calcium, phosphorus, bone calcium content, and histopathological were detected and observed. Results Serum calcium and phosphorus in ovariectomized rats had no significant change compared to control group. Serum calcium and phosphorus in calcium gluconate group, Jingui shenqi wan group, and Jingui shenqi wan plus calcium gluconate group had no significant change compared to control group, either. Bone calcium content in ovariectomized rats significantly decreased compared to control rats. Bone calcium content in calcium group, Jingui shenqi wan, and combination group was significantly improved compared to control group, and the combination group improved significantly （P 〈 0. 01 ） . Histopathological observation showed that in the cortical bone the encroached changes area was smaller, and the bone trabecula were thinner and arranged consistently in calcium group and Jingui shenqi wan group. In Jingui shenqi wan plus calcium gluconate group, the thickness of cortical bone was normal, and the bone trabecula were slightly thinner and arranged more regularly. Conclusion Jingui shenqi wan plus calcium gluconate treatment reduced the loss of bone minerals in ovariectomy-induce osteoporosis in rats, thus had beneficial effect on prevention and treatment of osteoporosis.