共聚焦显微镜在真菌性角膜炎中的应用

Application of confocal microscopy in fungal keratitis

目的:探讨共聚焦显微镜在真菌性角膜炎中的应用价值。方法:回顾性分析在我院就诊的拟诊为真菌性角膜炎患者42例42眼,行共聚焦显微镜检查,同时行角膜病灶组织刮片染色检查。结果:真菌性角膜炎患者42例42眼,行共聚焦显微镜检查,观察到真菌菌丝为39例39眼,检出阳性率93%(39/42),行角膜病灶组织刮片染色镜检,观察到真菌菌丝为30例30眼,检出阳性率71%(30/42)。统计学分析表明两者差异有统计学意义(χ2=6.574,P<0.05)。结论:共聚焦显微镜是一种无创、高效、直观的检查设备,对真菌性角膜炎的初步诊断有着重要的临床价值。

AIM：To investigate the application of confocal microscopy in fungal keratitis.METHODS：A total of 42 patients 42 eyes diagnosed with suspected fungal keratitis were performed the confocal microscopy and corneal lesions stained tissue smear examination simultaneously and the results were analysed retrospectively.RESULTS：In 42 patients 42 eyes with fungal keratitis,fungal hyphae was observed in 39 eyes（93%） by the confocal microscopy,however in 30 eyes（71%） by corneal lesions stained tissue smear examination.Statistical analysis showed that the difference was statistically significant（χ 2=6.574,P0.05）.CONCLUSION：Confocal microscopy is a noninvasive,efficient,intuitive inspection equipment,which has important clinical value in the initial diagnosis of fungal keratitis.