斛芪浸膏对Wistar大鼠涎腺辐射损伤的保护作用

Protective effects of Chinese herbal medicine Huqi extractum on salivary glands against radiation in Wistar rats

目的:探讨中药复方斛芪浸膏对Wistar大鼠涎腺辐射损伤的保护作用。方法:100只Wistar大鼠随机分为假照射组,模型组和高、中、低剂量斛芪浸膏组。除假照射组大鼠外,采用60Coγ射线15Gy单次局部照射大鼠头颈部建立涎腺辐射损伤模型。于灌胃3d及40d后,分别收集唾液,比色法检测唾液中K+、Na+含量,碘-淀粉酶比色法检测唾液淀粉酶活性,酶联免疫吸附测定法检测唾液中分泌型免疫球蛋白A(secretory immunoglobulinA,sIgA)含量;取涎腺组织苏木精-伊红染色检测涎腺形态改变。结果:用药3d后,各辐射组大鼠均出现涎腺辐射损伤,与假照射组比较,模型组及低、中剂量斛芪浸膏组唾液sIgA含量及体质量下降,唾液淀粉酶活性升高。涎腺组织病理切片显示,用药3d后各辐射组腺泡胞浆水肿,空泡形成,40d后涎腺实质细胞萎缩,数量减少。用药40d后,高剂量斛芪浸膏组大鼠唾液sIgA含量、淀粉酶活性及体质量接近假照射组;涎腺组织结构与假照射组差别不明显。结论:斛芪浸膏能促进涎腺辐射损伤大鼠腺泡结构和功能的恢复。

Objective：To explore the protective effects of Huqi extractum,a compound Chinese herbal medicine,on salivary glands against radiation in Wistar rats. Methods：One hundred Wistar rats were randomly divided into sham-exposure group,untreated group,and low-,medium-and high-dose Huqi groups. Local irradiation of 60Co gamma-rays with a single dose of 15 Gy was applied to the salivary glands of the Wistar rats except the sham-exposure group. After 3-and 40-day treatment,saliva was collected. Colorimetric method,iodine-amylase colorimetric method and enzyme-linked immunosorbent assay were used to detect concentrations of sodium （Na^＋）,potassium （K^＋） and secretory immunoglobulin A （sIgA） and activity of salivary amylase. Pathological changes of salivary gland tissues were observed by hematoxylin-eosin straining. Results：After 3-day administration,radiation-induced salivary gland injuries were obvious and prevalent in irradiated rats. Comparing with the sham-exposure group,saliva concentration of sIgA and body weight were reduced in other irradiated groups,except those in the high-dose Huqi group,while salivary amylase level was increased. At 3-day phase,pathologic changes of the salivary glands were featured as swelling acinus plasm and vacuolation. At 40-day phase,atrophy of gland cells was dominant. After 40-day administration,there were no significant differences between the high-dose Huqi group and sham-exposure group in sIgA and amylase levels and body weight,and according to the histological examination,no significant difference was revealed under the optical microscope.Conclusion：Chinese herbal medicine is helpful for the recovery of the salivary glands from the radiation injury,morphologically and functionally in rats