摘要
目的:探讨中药复方斛芪浸膏对Wistar大鼠涎腺辐射损伤的保护作用。方法:100只Wistar大鼠随机分为假照射组,模型组和高、中、低剂量斛芪浸膏组。除假照射组大鼠外,采用60Coγ射线15Gy单次局部照射大鼠头颈部建立涎腺辐射损伤模型。于灌胃3d及40d后,分别收集唾液,比色法检测唾液中K+、Na+含量,碘-淀粉酶比色法检测唾液淀粉酶活性,酶联免疫吸附测定法检测唾液中分泌型免疫球蛋白A(secretory immunoglobulinA,sIgA)含量;取涎腺组织苏木精-伊红染色检测涎腺形态改变。结果:用药3d后,各辐射组大鼠均出现涎腺辐射损伤,与假照射组比较,模型组及低、中剂量斛芪浸膏组唾液sIgA含量及体质量下降,唾液淀粉酶活性升高。涎腺组织病理切片显示,用药3d后各辐射组腺泡胞浆水肿,空泡形成,40d后涎腺实质细胞萎缩,数量减少。用药40d后,高剂量斛芪浸膏组大鼠唾液sIgA含量、淀粉酶活性及体质量接近假照射组;涎腺组织结构与假照射组差别不明显。结论:斛芪浸膏能促进涎腺辐射损伤大鼠腺泡结构和功能的恢复。
Objective:To explore the protective effects of Huqi extractum,a compound Chinese herbal medicine,on salivary glands against radiation in Wistar rats. Methods:One hundred Wistar rats were randomly divided into sham-exposure group,untreated group,and low-,medium-and high-dose Huqi groups. Local irradiation of 60Co gamma-rays with a single dose of 15 Gy was applied to the salivary glands of the Wistar rats except the sham-exposure group. After 3-and 40-day treatment,saliva was collected. Colorimetric method,iodine-amylase colorimetric method and enzyme-linked immunosorbent assay were used to detect concentrations of sodium (Na^+),potassium (K^+) and secretory immunoglobulin A (sIgA) and activity of salivary amylase. Pathological changes of salivary gland tissues were observed by hematoxylin-eosin straining. Results:After 3-day administration,radiation-induced salivary gland injuries were obvious and prevalent in irradiated rats. Comparing with the sham-exposure group,saliva concentration of sIgA and body weight were reduced in other irradiated groups,except those in the high-dose Huqi group,while salivary amylase level was increased. At 3-day phase,pathologic changes of the salivary glands were featured as swelling acinus plasm and vacuolation. At 40-day phase,atrophy of gland cells was dominant. After 40-day administration,there were no significant differences between the high-dose Huqi group and sham-exposure group in sIgA and amylase levels and body weight,and according to the histological examination,no significant difference was revealed under the optical microscope.Conclusion:Chinese herbal medicine is helpful for the recovery of the salivary glands from the radiation injury,morphologically and functionally in rats
出处
《中西医结合学报》
CAS
2011年第1期70-76,共7页
Journal of Chinese Integrative Medicine
基金
国家科技重大专项项目(No.2008ZXJ09001-018)
关键词
益气养阴
中草药
辐射防护
涎腺
大鼠
supplementing qi and nourishing yin
Chinese herbal medicine
radiation protection
salivary gland
rats