泛素结合酶-10表达对结直肠癌细胞增殖的影响

Effects of UbcH10 expression on proliferation of colorectal cancer cells

目的探讨泛素结合酶-10(Ubiquitin-conjugating enzymes,UbcH10)表达对结直肠癌细胞增殖的影响。方法采用脂质体LipofectamineTM2000转染人UbcH10的真核表达载体pcDNA3.1-UbcH10及其对照载体pcDNA3.1至结直肠癌细胞LoVo,蛋白印迹(Western blot)技术分析UbcH10的表达;细胞增殖试验(CCK-8试剂)检测UbcH10表达对LoVo细胞增殖的影响;流式细胞术检测UbcH10表达对LoVo细胞周期的影响。结果 Westernblot分析证实,与对照组细胞相比,转染重组质粒的LoVo细胞中UbcH10表达明显升高;细胞生长增殖曲线显示,UbcH10过度表达的细胞生长明显快于对照组细胞。流式细胞术分析细胞周期结果显示:与对照组相比,UbcH10过度表达组细胞在G2-M期分布明显减少(P<0.05)。结论 UbcH10能促进结直肠癌细胞增殖,调控细胞周期进展可能是其潜在的作用机制。

Objective To investigate the effect of the UbcH10（Ubiquitin-conjugating enzymes） expression on the proliferation of colorectal cancer cells.Methods The pcDNA3.1-UbcH10 and pcDNA3.1 were transfected into LoVo cells.UbcH10 expression was determined by Western blot.Proliferation of LoVo cells was detected by CCK-8 kit.The effect of UbcH10 on the cell cycle of colorectal cancer cells was assessed by flow cytometry.Results The UbcH10 expression in LoVo cells transfected with pcDNA3.1-UbcH10 was more obviously increased than that in control cells.Over-expression of UbcH10 obviously promoted cell proliferation compared with the controls.UbcH10 over-expression significantly decreased cell cycle distribution in the G2-M phase（P 〈0.05）.Conclusion UbcH10 can promote proliferation of colorectal cancer cells.The potential mechanism may depend on its regulation of the cell cycle.