G-CSF联合VEGF增加APP转基因小鼠AD模型海马CD133及SYN的表达

G-CSF and VEGF increase expressions of CD133 and synaptophysin in the APP transgenic mouse model

目的观察粒细胞集落刺激因子(G-CSF)联合血管内皮生长因子(VEGF)对APP转基因AD小鼠模型突触可塑性的影响。方法 54只10月龄APP转基因小鼠随机分为G-CSF联合VEGF干预组(联合组)、G-CSF干预组及对照组3组。联合组:腹腔注射VEGF(8μg/kg.d)连续3d,之后皮下注射G-CSF(50μg/kg.d),连续7d。G-CSF干预组:腹腔注射PBS连续3d,之后皮下注射G-CSF(50μg/kg.d),连续7d。对照组:腹腔注射PBS,连续3d,之后皮下注射生理盐水,连续7d。各组分别于给药后7、14、28d利用免疫组织化学检测海马区CD133,免疫荧光双标记技术检测5溴-2脱氧尿核苷(B rdU)和突触素(SYN)的双表达。结果联合组较G-CSF干预组及对照组CD133和SYN表达明显增加。结论 G-CSF联合VEGF能促进神经再生,增强突触功能,改善突触可塑性。

Objective To observe effects of G-CSF and VEGF on synaptic plasticity in the APP transgenic mouse model.Methods 54 10-month-old APP transgenic mice were randomly divided into 3 groups：the G-CSF combined with VEGF group,the G-CSF group and the control group.Injections of VEGF（8 μg/kg·d） for 3 d were peritoneally given to mice in the G-CSF combined with VEGF group,then injections of G-CSF（50 μg/kg·d） for 7 d were subcutaneously given;injections of PBS for 3 d were peritoneally given to mice in the G-CSF group,then injections of G-CSF（50 μg/kg·d） for 7 d were subcutaneously given;injections of PBS for 3 d were peritoneally given to mice in the control group,then injections of normal saline for 7 d were subcutaneously given.Expression of CD133 was observed 7,14 and 28 days after administration by immunohistochemistry.BrdU and synaptophysin（SYN） double expressions were detected by fluorescent staining,and influences of G-CSF combined with VEGF on proliferation of mouse stem cells and synaptic plasticity were observed.Results Expressions of CD133 and SYN increased in the G-CSF combined with VEGF group（P 〈0.05）.Conclusion G-CSF combined with VEGF may increase synaptic function and improve synaptic plasticity by promoting neurogenesis.