子宫内膜异位症在位内膜中神经纤维的表达

Expression of nerve fibers in the eutopic endometrium in endometriosis

目的探讨子宫内膜异位症（内异症）在位内膜中神经纤维的表达。方法 2008年1月2009年12月检查确诊为内异症的患者共64例,按美国生育协会修正分期（rAFS）分为4组,Ⅰ期11例（1组）、Ⅱ期12例（2组）、Ⅲ期20例（3组）、Ⅳ期21例（4组）,手术确诊无内异症者10例作为阴性对照组（0组）。通过诊断性刮宫取得的子宫内膜组织分别用神经标志物生长相关蛋白-43（GAP-43）及S-100进行免疫组化测定,比较4组内异症者及非内异症者的在位内膜神经纤维密度及内异症者的神经纤维与内膜腺体的最近距离。结果 rAFS分期各组及对照组的神经纤维密度的差异有统计学意义（P均〈0.001）;4组内异症者的神经纤维与内膜腺体的最近距离差异无统计学意义（P均〉0.05）。结论内异症病情进展与在位内膜神经纤维密度密切相关,与神经纤维及内膜腺体间的距离无相关性。

Objective To investigate expression of nerve fibers in the eutopic endometrium in endometriosis.Methods 64 patients diagnosed with endometriosis from January 2008 to December 2009 in our hospital were divided into four groups according to the revised American Fertility Society scoring system（rAFS）： and stageⅠ,11 cases（group 1）;stageⅡ,12 cases（group 2）;stage Ⅲ,20 cases（group 3）;and stage Ⅳ,21 cases（group 4）.10 patients diagnosed without endometriosis served as the controls（group 0）.Nerve fibers in endometrium tissue sections by diagnostic uterine curettage were immunohistochemically detected using growth associated protein 43（GAP-43） and S-100.Nerve fiber density in the eutopic endometrium was compared between patients with and without endometriosis,and the nearest distance between nerve fibers and endometrial glands in the endometriosis groups was compared.Results Nerve fiber densities in the endometriosis groups and the control group were significantly different（both GAP-43 groups and S-100 groups,P 〈0.001）.Nearest distances between nerve fibers and endometrial glands in the endometriosis groups had no difference（both GAP-43 groups and S-100 groups,P 〉0.05）.Conclusion Nerve fiber density in the eutopic endometrium is close related to progression of endometriosis.Progression of endometriosis is not related to the nearest distance between nerve fibers and endometrial glands.