摘要
目的研究转化生长因子-β1(TGF-β1)对体外培养早产鼠的肺成纤维细胞(LFs)结缔组织生长因子(CTGF)基因表达的影响。方法以孕19dWistar大鼠剖宫取出的仔鼠为对象,原代培养LFs,实验组分别采用不同浓度(1ng/mL、5ng/mL、10ng/mL)和不同作用时间(12h、24h、48h)TGF-β1刺激LFs,对照组无血清培养基培养(即0ng/mL)。应用RT-PCR方法检测LFs的CTGFmRNA表达。结果与对照组比较,实验组LFs的CTGFmRNA水平增高(P<0.05),且随TGF-β1作用浓度的增加、作用时间的延长CTGFmRNA表达逐渐上升,差异有统计学意义(P<0.05)。结论 TGF-β1能刺激肺成纤维细胞中CTGF基因表达,并且以剂量和时间依赖方式上调CTGF基因表达。
Objective To study the effects of transforming growth factor-β1（TGF-β1） on the gene expression of connective tissue growth factor（CTGF） in cultured lung fibroblasts of embryonic rats in vitro.Methods Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts（LFs）.The cells in the experimental group were treated by different concentrations（1,5 or 10 ng/mL） and different durations（12,24 or 48 hrs） of TGF-β1 to stimulate the LFs.The cells in the control group were cultured in serum-free medium.RT-PCR method was applied to detect CTGF mRNA expression in LFs.Results Compared with the control group,the levels of CTGF mRNA in LFs in the experimental group increased significantly（P0.05）.CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment（P0.05）.Conclusions TGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2011年第1期36-39,共4页
Chinese Journal of Contemporary Pediatrics
基金
国家自然科学基金(No.30872781)
