利用Micro-PET显像技术分析阿尔茨海默病小鼠模型的脑糖代谢

Analysis of cerebral glucose metabolism in mouse of Alzheimer’s disease model by Micro-PET

目的利用小动物PET(Micro-PET)影像技术18氟-脱氧葡萄糖(18 F-FDG)摄取率分析阿尔茨海默病(AD)小鼠模型的脑糖代谢,探索评价治疗AD药物的影像分析技术。方法正常对照组、APPswe/PSΔE9转基因模型组和安理申[2 mg/(kg.d)]治疗组小鼠共9只,自腹腔注射放射性示踪剂18F-FDG,采集脑部Micro-PET图像,通过软件IRW计算并比较各组小鼠大脑与额叶、颞叶感兴趣区(ROI)每克组织18 F-FDG的摄取率。结果利用18F-FDG的Micro-PET影像分析可以检测AD小鼠模型的脑糖代谢,并发现APPswe/PSΔE9转基因小鼠的脑糖摄取明显降低,与人类AD患者表现的脑糖摄取降低一致,安理申治疗可以提升APPswe/PSΔE9转基因小鼠的脑糖摄取。结论 18F-FDG Micro-PET影像分析可以用于小鼠AD模型脑葡萄糖代谢水平的检测,并可以作为阿尔茨海默病治疗药物提升脑糖代谢作用的分析技术。

Objective To evaluate the cerebral glucose metabolism of Alzheimer＇ s disease（AD） model mouse by 18F-FDG and Micro-PET, and develop an imaging technique to evaluate AD drugs therapeutic effect on animal models. Methods The 9 mice of control group, APPswe/PS△E9 double-transgenic group and aricept treatment group[ 2 mg/（ kg. d） ] were injected 18F-FDG by peritoneal cavity and scanned by Micro-PET. The glucose uptake value of cerebrum,frontal cortex and temporal cortex region of interest（ ROD were analysed by IRW software. Results The images of 18F-FDG and Micro-PET could be used to detect the cerebral glucose metabolism of AD mouse model. We found that the cerebral glucose metabolism was decreased in the APPswe/PS△E9 double-transgenic mice, which was consistent with phenotype of the human AD. Aricept treatment improved the cerebral glucose metabolism of AD disease model mice. Conclusion The imaging analysis using 18F-FDG and Micro-PET could be used to detect the cerebral glucose metabolism of AD mouse model which could be used as a useful technique for evaluating the