涎腺黏液表皮样癌中Cox-2、Ki-67的表达及意义

Expression of Cox-2 and Ki-67 in mucoepidermoid carcinoma of salivary glands and its significance

目的检测涎腺黏液表皮样癌(mucoepidermoid carcinoma,MEC)、正常涎腺组织(normal salivary gland,NSG)中Cox-2、Ki-67蛋白的表达情况,为MEC预后评估提供可筛选的标志物。方法应用免疫组化染色方法(PV-6001二步法)检测11例NSG、34例MEC中Cox-2、Ki-67的表达,采用SPSS 16.0软件对结果进行统计学分析。结果 MEC、NSG中Cox-2的表达差异有统计学意义(P<0.01);在不同病理类型(高、中、低分化)、是否复发、是否转移MEC中Cox-2的表达差异有统计学意义(P<0.05,P<0.01),而在不同性别、年龄、民族、部位比较差异无统计学意义。Ki-67在MEC与NSG中表达差异有统计学意义(P<0.01),在高、中与低分化间、不同部位、是否复发、是否转移中的表达差异有统计学意义(P<0.05,P<0.01);在高、中分化间及不同性别、年龄、民族中无统计学意义。Cox-2和Ki-67在MEC中表达正相关(rs=0.788,P<0.01)。结论涎腺黏液表皮样癌Cox-2、Ki-67的表达与组织病理分型有关,可能参与MEC的发生、发展过程,二者协同表达越高越容易发生浸润和远处转移;联合检测Cox-2、Ki-67可能有助于MEC的预后判断。

Objective To observe the expression of Cox-2 and Ki-67 protein in mucoepidermoid carcinoma(MEC) and normal salivary gland(NSG),so as to search for biomarkers for evaluation of MEC prognosis.Methods The expression of Cox-2 and Ki-67 protein was examined by PV-6001 staining in 11 NSG and 34 MEC samples,and the experimental data were statistically analyzed by SPSS 16.0 software.Results The expressions of Cox-2 were significantly different between MEC and NSG samples(P < 0.01).Cox-2 expressions were also significantly different between MEC samples with different differentiation,metastasis and recurrent statuses(P < 0.05,P < 0.01),and were similar between those with different sexes,ages,or ethnicities(P > 0.05).The expressions of Ki-67 were significantly different between MEC and NSG samples(P < 0.01).Ki-67 expressions were also significantly different between MEC samples of high-and moderate-differentiation degrees with low-differentiation degrees,and samples with different recurrence,and metastasis statuses and sites(P < 0.05,P < 0.01),and were similar between the high-differentiation and middle-differentiation samples,and samples with different sexes,ages,and ethnicities.Cox-2 expression was positively correlated with Ki-67 expression in MEC samples(rs = 0.788,P < 0.01).Conclusion Cox-2 and Ki-67 expressions in MEC are associated with the pathological types of MEC,and they may participate in the development and progression of MEC.High expression of both is associated with local infiltration and distant metastasis.Co-detection of Cox-2 and Ki-67 is helpful for predication of MEC prognosis.