曲美他嗪对大鼠心肌缺血-再灌注损伤的保护作用

The protective effect of trimetazidine on the myocardial ischemia-reperfusion injury in rats

目的探讨曲美他嗪(TMZ)对缺血-再灌注(I-R)后大鼠心肌细胞凋亡的保护作用。方法 SD大鼠随机分为假手术组(A组)I、-R组(B组)、低浓度TMZ(1 ml/100 g)组(C组)和高浓度TMZ(3 ml/100 g)组(D组),每组6只。制备大鼠心肌I-R模型,采用流式细胞仪(FCM)、原位末端标记(TUNEL)技术、免疫组化检测心肌组织细胞凋亡情况,并观察细胞形态变化。结果 B组细胞凋亡指数、TUNEL阳性细胞数、Bcl-2、Bax较A组增高[(0.2416±0.0263)vs.(0.0469±0.0136),(3.213±0.14)vs.(0.44±0.133),(2.057±0.187)vs.(0.47±0.082),(4.257±0.135)vs.(0.52±0.058)(P<0.05)],而C、D组细胞凋亡指数、TUNEL阳性细胞数、Bax明显下降(P<0.05),D组减低更明显。结论 TMZ可抑制心肌I-R损伤中的细胞凋亡。

Objective To investigate the protective effect of trimethazine（TMZ） on rat myocardial cell apoptosis after ischemia-reperfusion（IR）.Methods Twenty-four SD rats were randomly and equally divided into 4 groups of A（sham operated）,B（I-R model）,C（treated with TMZ 1ml/100g） and D（treated with TMZ 3ml/100g）.The myocardial I-R model in rats was established.The myocardial cell apoptosis was respectively detected by flow cytometry（FCM）,TUNEL technology and immunohistochemistry,and the changes of cell morphology were observed by microscope.Results The apoptotic index,the positive cell number and the levels of Bcl-2 and Bax were higher in group B than those in group A[（0.2416±0.0263） vs.（0.0469±0.0136）,（3.213±0.140） vs.（0.440±0.133）,（2.057±0.187） vs.（0.470±0.082）,（4.257±0.135） vs.（0.520±0.058）]（P0.05）,which were much lower in groups of C and D（P0.05）.Conclusion TMZ can inhibit cell apoptosis during myocardial I-R,which in turn attenuates I-R injury.