FQ-PCR检测胃癌患者外周血C-erbB-2 mRNA的表达及其临床意义

Expression and clinical significance of C-erbB-2 mRNA expression in peripheral blood of patients with gastric cancer

目的探讨原癌基因人表皮生长因子2(C-erbB-2)在胃癌患者外周血中的mRNA表达,并评价其在诊断胃癌患者外周血微转移及与临床病理因素的相关性和意义。方法采用实时荧光定量PCR(FQ-PCR)法检测56例经病理学确诊的胃癌患者、20例慢性胃炎患者和30例健康对照者外周血C-erbB-2和内参基因3-磷酸甘油醛脱氢酶(GAPDH)的表达水平,分析组间相对表达水平(C-erbB-2/GAPDH)的差异及与临床病理因素的关系。结果胃癌组C-erbB-2 mRNA阳性表达率高于慢性胃炎和健康对照组(26.8%vs.0%和0%)(P<0.01)。阳性表达率与患者年龄、性别无关,而与肿瘤分化程度、有无淋巴结转移、TNM分期和浸润深度相关(P<0.05或P<0.01)。结论检测胃癌患者外周血中C-erbB-2 mRNA的表达对诊断早期胃癌及微转移状况是一种敏感且特异的方法。

Objective To explore the expression level of human epidermal growth factor-2（C-erb-B2） mRNA in peripheral blood of patients with gastric cancer and evaluate its clinical value in diagnosing the micro-metastatic status and its correlation with clinicopathological parameters.MethodsFQ-PCR was used to determine the expression level of C-erbB-2 mRNA and glyceraldehyde-3-phosphate dehydrogenase（GAPDH） mRNA in peripheral blood of 56 cases with gastric cancer（group A）,20 cases with chronic gastritis（group B） and 30 healthy volunteers（group C）.ResultsThe positive expression rate of group A was significantly higher than that of group B or group C（26.8% vs.0% or 0%）（P0.01）,which was correlated to state of differentiation,lymph node metastasis,clinical TNM stage,and depth of invasion（P0.05 or P0.01） but not correlated to age and gender.ConclusionDetecting the expression level of C-erbB-2 mRNA in peripheral blood of patients with gastric cancer is a sensitive and specific method for diagnosing the early stage of tumor and micro-metastasis.