细胞间黏附分子-1基因K469E多态性与类风湿性关节炎的关系

No association of K469E polymorphism of intercellular adhesion molecule-1 with rheumatoid arthritis

目的探讨细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）基因K469E多态性与类风湿性关节炎（rheumatoid arthritis，RA）的关系。方法对275例类风湿性关节炎患者和254名体检健康者作为对照组进行研究。采用聚合酶链反应限制性片段长度多态性方法分析ICAM-1基因K469E的多态性。结果RA组K469E位点KK、KE和EE基因型频率为0．535、0．411和0．054；健康对照组K469E位点KK、KE和EE基因型频率为0．512、0．437和0．051。RA组K469E基因型频率与健康对照组相比差异无统计学意义（X^2=0．371，P=0．831）。RA组K等位基因频率（0．74）与健康对照组（0．73）相比差异无统计学意义（X^2=0．127，P=0．721，OR=1．051，95％CI为0．800～1．381），在RA组中KK＋KE基因型频率与对照组相比，差异无统计学意义（P=0．863，0R=0．935，95％CI为0．436～2．005）。结论 ICAM-1基因K469E多态性分布与RA的易感性无明显相关性。

Objective To investigate the association of the intercellular adhesion molecule 1 gene （ICAM-1）K469E polymorphism and rheumatoid arthritis （RA）. Methods Two hundred and seventy five patients with RA and 254 healthy individuals were collected and enrolled in the study. The K469E polymorphism of ICAM-1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results The genotype frequencies of KK, KE and EE of K469E polymorphism were 0. 535,0. 411 and 0. 054 respectively in the RA patients, and 0. 512,0. 437 and 0. 051 respectively in the healthy individuals, and there was no significant difference between the two groups （X^2= 0. 371,P=0. 831）. The frequencies of the K469 allele were 0. 74 and 0. 73 in the RA patients and the controls respectively （X^2 = 0. 127, P= 0. 721, OR= 1. 051,95 % CI： 0. 800-1. 381 ）. No significant difference was observed in KK＋KE genotype frequencies between the two groups （P=0. 863）, with an odds ratio of 0. 935 （95% CI： 0. 436- 2. 005）. Conclusion The K469E polymorphism of the ICAM1 gene was not associated with the susceptibility of rheumatoid arthritis.