摘要
目的:探讨可溶性血管内皮生长因子受体(sFlt-1)与原因不明复发性流产(URSA)的相关性。方法:①采用酶联免疫吸附试验法(ELISA)测定30例原因不明复发性流产胚胎停育患者(URSA组)和30例正常早孕孕妇(对照组)血清sFlt-1水平;②采用逆转录聚合酶链反应(RT-PCR)方法测定各组孕妇绒毛组织sFlt-1mRNA表达水平。结果:复发性流产胚胎停育组与正常早孕对照组血清sFlt-1水平分别为13796.67±11917.08ng/L和3540.67±2989.80ng/L;复发性流产胚胎停育组与正常早孕对照组绒毛组织sFlt-1mRNA表达水平两组计量资料不符合正态分布,用Mann-Whitney秩和检验比较,RSA组sFlt-1mRNA均秩为41.37,正常组sFlt-1mRNA均秩为19.63,P<0.01,组间差异非常显著性意义(P<0.01)。结论:sFlt-1表达异常与复发性流产具有相关性,其表达水平升高可能参与RSA发生的病理生理过程。
Objective：To investigate the expression of soluble fins-like tyrosine kinase receptor 1（sFlt-1） in unexplained recurrent spontaneous abortion and（URSA） to analyze the relationship between them.Methods：Thirty patients with URSA and 30 normal early pregnant women（the control） were included in this study.The level of sFlt-1 in serum was explored by enzyme-linked immunosorbent assays（ELISA）.Expression level of sFlt-1 mRNA in chorionic villus tissue was detected by quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）.Results：The mean serum value of sFlt-1 in serum was significantly higher in women with URSA than in the control（13 796.67±11 917.08 ng/ml vs 3 540.67±2 989.80 ng/ml,P0.01 by t test）.The expression level of VEGF mRNA and sFlt-1 mRNA in chorionic villus tissue in URSA group was statistically higher than that in the control（P0.01,by Mann-Whitney test）.Conclusion：There is a relationship between sFlt-1 and RSA.The high expression of sFlt-1 may be involved in the pathogenesis of URSA.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2011年第1期58-61,共4页
Reproduction and Contraception
基金
广西自然科学基金资助项目(桂科回0991008)
关键词
可溶性血管内皮生长因子受体(sFlt-1)
原因不明复发性流产(uRSA)
ELISA
RT-PCR
soluble fms-like tyrosine kinase receptor 1（sFlt-1）
unexplained recurrent spontaneous abortion（URSA）
enzyme-linked immunosorbent assays（ELISA）
quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）
