一种工程菌的高酶活及其固定化细胞对农药的降解

High enzyme activity of an engineered bacteria and degradation of pesticides by immobilized cells

将抗性尖音库蚊五带亚种的执有机磷农药基因—酯酶基因克隆到质粒pRL－439中．经检测，重组质粒中的酯酶基因已得到表达，获得高酶活的工程菌．将工程菌固定化后对两类难降解农药（有机氯、菊酯类）进行降解，结果表明，固定化细胞在1h内对上两类农药可降解90％以上，具有很高的降解效力．

Culex pipiens quinquefasciatus, a gene inducing resistance to organophosphate pesticide was cloned into plasmid pRL-439. Expression of recombinant plasmid containing esterase gene was detected. An engineered bacterium possessing high enzyme activity was obtained and immobilized. Assays showed that more than 90% of either acetofenate (7504 an organic chloride pesticide) or pyrethroids was degraded by the immobilized cells within one hour.