大肠杆菌中利用苏云金芽胞杆菌强启动子p1Ac指导Cry1Ac蛋白表达的特性分析

The Nature Analysis of Expressed Cry1Ac Protein Initiated by Strong Promoter p1Ac from Bacillus thuringensis in E.coli

对利用苏云金芽胞杆菌(Bacillus thuringiensis,Bt)强启动子——cry1Ac基因启动子p1Ac指导cry基因在大肠杆菌中的表达进行了研究。结果显示,大肠杆菌中由启动子p1Ac指导表达的Cry1Ac蛋白与苏云金芽胞杆菌来源的Cry1Ac蛋白在碱溶性、胰蛋白酶活化、杀虫活性等方面有较好的一致性,从而解决了目前商业化载体大肠杆菌表达cry基因时形成不易溶解的包涵体问题。同时,还对p1Ac指导的cry1Ac基因在大肠杆菌中表达的发酵条件进行了初步探索。

This study was on the expression of Cry1Ac protein in E.coli initiated by the promoter of cry1Ac gene（p1Ac）from Bacillus thuringiensis（Bt）.The results showed that there are some similarities between Cry1Ac protein initiated by promoter p1Ac in E.coli and Cry1Ac protein from Bt on alkali soluble nature,trypsin activated nature and toxicity.This method solved the problem that commercial expression vectors for cry gene expression are susceptible to form insoluble inclusion bodies.Meanwhile,we analyzed the fermentation condition that Cry1Ac expression initiated by p1Ac.