柿ζ-胡萝卜素脱氢酶基因的克隆与序列分析

Cloning and Sequence Analysis of ζ-Carotene Desaturase Gene in Persimmon(Diospyros kaki L.)

通过对柿ζ-胡萝卜素脱氢酶(-ζCarotene desaturase,ZDS)基因的克隆及序列分析,旨在为改良柿果实品质奠定基础。根据GenBank中其他植物ZDS基因的保守序列设计了两条PCR扩增引物,以柿果实中所提取的RNA为模板,采用RACE技术扩增出一条约为500bp的条带。经过序列分析,其长578bp,不含内含子,具有部分开放阅读框(177bp)、终止密码子(TGA)和poly(A)尾巴(11bp),共编码58个氨基酸。该序列及其所编码的氨基酸与其他植物ζ-胡萝卜素脱氢酶基因均具有较高的同源性。该基因已提交GenBank数据库,登录号为GU075728。

To lay the foundation for improving the quality of persimmon fruit,ζ-carotene desaturase（ZDS）gene was cloned from persimmon.Two PCR primers were designed according to conservative sequence of ZDS gene in other plants reported in GenBank.Total RNA of persimmon fruit as template,a 500 bp fragment was amplified according to RACE technology.Sequence analysis showed that the fragment was 578 bp and encoded 58 amino acids.Homology analysis showed that both nucleotide and amino acids sequence shared high similarities with ZDS genes cloned from other plants.This gene had been submitted to GenBank database with GU075728.