巨噬细胞移动抑制因子在膀胱尿路上皮癌组织中的表达及其临床意义

Expression and significance of mcrophage migration inhibitory factor in bladder urothelial cell carclnoma

目的 探讨膀胱尿路上皮癌组织中巨噬细胞移动抑制因子（MIF）的表达及其临床意义.方法 应用免疫组织化学染色方法,研究110例膀胱尿路上皮癌和10例正常膀胱黏膜组织中MIF的表达,并分析其与临床病理特征及预后的关系.结果 MIF在正常膀胱黏膜组织中无或弱表达.110例膀胱尿路上皮癌组织肿瘤细胞浆内MIF阳性表达率为65.5%（72/110）,其中27例（24.5%）在细胞核中也有表达.非肌层浸润性膀胱尿路上皮癌中MIF表达水平高于肌层浸润性膀胱尿路上皮癌（P=0.001）.肿瘤细胞核内MIF表达与肿瘤病理分级（P=0.034）、临床病理分期（P=0.028）和肿瘤大小（P=0.002）均呈负相关.细胞核内MIF表达水平与患者无瘤生存明显相关（P=0.042）,MIF阳性组患者有较长的无瘤生存时间.结论 非肌层浸润性膀胱尿路上皮癌中MIF表达水平高于肌层浸润性膀胱尿路上皮癌,细胞核内MIF表达可能预示膀胱尿路上皮癌患者预后良好.

Objective To evaluate the expression and clinical significance of macrophage migration inhibitory factor （MIF） in patients with bladder urothelial cell carcinoma.Methods Immunohistochemical staining for MIF was performed on tissue sections of 110 patients with bladder urothelial cell carcinoma and 10 normal controls, and the correlations between MIF and clinicopathological characteristics and prognosis were also analyzed.Results Normal bladder urothelium from control subjects showed negative or weak staining of MIF.Of the cancer specimens, 72/110 （65.5%） showed a moderate to strong staining of MIF.The expression of MIF protein was found predominantly in the tumor cell cytoplasm and inversely correlated with tumor stage.27 cases also showed a positive intranuclear staining of MIF, which was inversely correlated with tumor grade, stage and tumor size.Kaplan-Meier analysis showed that the expression of MIF in the cell nuclei was associated with disease-free survival for the cancer patients, but multivariate analysis showed that MIF was not an independent prognostic factors.Conclusions The expression of MIF in nonmuscle invasive bladder cancer tissues was more frequently than that in muscle-invasive disease, the positive staining of MIF in cell nuclei might be a favorable biomarker for patients with bladder urothelial cell carcinoma.