硬骨鱼紧张素ⅡT21M基因多态性与东乡族原发性高血压关系的研究

Association of Urotensin Ⅱ T21M polymorphisms with essential hypertension from Dongxiang nationality

目的:研究硬骨鱼紧张素ⅡT21M基因(UTS2-T21M)单核苷酸多态性(SNP)与东乡族原发性高血压(EH)的关系。方法:用PCR-RFLP及电泳分析法进行基因分型;SPSS软件分析各基因型与东乡族人群EH的相关性。结果:研究包括120例东乡族EH患者及102例正常人。EH患者与对照组间在基因型,等位基因频率上均有显著差异(P=0.021,0.014)。UTS2-T21M多态性,TT,TM,MM基因型的频率在病例组中分别40.0%,43.3%,16.7%;对照组相应基因型的频率为22.5%,55.0%,22.5%。T,M等位基因频率在病例组中分别61.7%,38.3%,对照组相应的等位基因频率为50.0%,50.0%。结论:UTS2-T21M基因多态性与东乡族人群EH相关。

Objective：To study the association of UTS2-T21M SNP with essential hypertension in Dongxiang nationality.Method：Genotyping of UTS2-T21M was conducted with PCR-RFLP and agarose gel electrophoreticanalysis.All statistical analyses were performed with SPSS.Result：120 essential hypertension（EH） cases and 102 healthy controls were studied.We found significant difference in genotypic and allelic frequency of T21M SNP in UTS 2 between EH patients and controls.（P=0.021,and 0.014,respectively）.Frequencies of TT,TM,MM Genotypes were 40.0%,43.3%,16.7% in case,And were 22.5%,55.0%,22.5% in controls;Frequencies of T,M allele were 61.7%,38.3% in case and 50.0%,50.0% in controls.Conclusion：Genetic variants of T21M of UTS2 were association with essential hypertension in Dongxiang population.