胰岛素样生长因子-Ⅰ活化组织工程骨修复骨缺损的实验研究

IGF-Ⅰ activated tissue engineered bone complex repairs bone defect in rabbits

目的探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对羟基磷灰石(HA)及自体红骨髓(ABM)复合物成骨诱导活性的作用。方法在36只兔桡骨中1/3上制造骨缺损模型后,按随机数字表法分为4组,每组9只,空白组缺损不做任何处理,HA/ABM组、IGF-Ⅰ活化HA组、IGF-Ⅰ活化HA/ABM组组织工程骨分别植入骨缺损处。术后于不同时间点分别行血清碱性磷酸酶(ALP)活性测定、扫描电镜检测评价成骨情况。结果①血清ALP活性:2、4、8、12周时,IGF-Ⅰ/HA/ABM组血清ALP活性显著高于其他各组(P<0.05),且其他组间比较有统计学意义(P<0.05)。②扫描电镜结果:12周时,IGF-Ⅰ/HA/ABM组仅见少量材料,骨缺损区被成熟板层状骨组织充填,材料与正常骨质间已分不清界限,骨缺损大部分修复,其成骨作用明显优于各组。结论 IGF-Ⅰ对以HA为支架材料组织工程骨的成骨诱导作用明显增强。

Objective To investigate the osteoinductive effect of insulin-like growth factor-Ⅰ（IGF-Ⅰ） in tissue engineered bone complex of hydroxyapatite（HA）/autogenous red bone marrow（ABM）.Methods The animal model of bone defect of one thirds bilateral radius was made on 36 rabbits.The animals were randomly divided into 4 groups.Their defects were repaired with no implant for the blank control group,with HA/ABM implant for HA/ABM group,with implant 400 ng/ml IGF-Ⅰ pre-added HA for IGF-Ⅰ/HA group,and with IGF-Ⅰ/HA/ABM implant for IGF-Ⅰ/HA/ABM group.Gross observation,X-ray examination and scanning electronic microscopy were used to study the repair of defect before and in 1,2,4,8 and 12 weeks after the repairing.Serum level of alkaline phosphatase（ALP） was detected.Results Serum level of ALP was significantly higher in IGF-Ⅰ/HA/ABM group than in other groups in 2,4,8 and 12 weeks after repairing（P0.05）,and the difference of serum ALP had statistical significance among these groups（P0.05）.Scanning electronic microscopy showed that there was a few amount of complex in the defect of IGF-Ⅰ/HA/ABM group in 12 weeks,and the defect was filled by mature lamellar bone which having no distinguished difference with normal bone.Better repairing was seen in IGF-Ⅰ/HA/ABM group than in other groups.Conclusion IGF-Ⅰ exerts significantly osteoinductive effect on tissue engineering bone of HA as scaffold material.